KARPAS-25

6072601, human lung (effusion), Round to polygonal cells growing singly in suspension

• 同義詞: KARPAS 25, KARPAS, 25, KARPAS25
• 分類程式碼代碼: 41106514

屬性

• 產品名稱: KARPAS-25, 06072601
• 生物源: human lung (effusion)
• 包裝: tube of 5 μg 06072601-DNA-5UG; pkg of vial of cells 06072601-1VL
• 增長模式: Suspension
• 染色體組型: Hyperdiploid with complex aberrant chromosomes
• 形態學: Round to polygonal cells growing singly in suspension
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice

描述

細胞系來源

Human acute plasma cell leukaemia

細胞系描述

Established in 1989 from the pleural effusion of a 27-year old woman diagnosed with acute plasma cell leukaemia who failed to respond to treatment and died within 3 weeks.

培養基

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

例行更新培養

Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 10⁵ cells/ml; 5% CO₂; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 10⁵ cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 10⁵ cells/ml, this tends to equate to a 1:3 split every 2-3 days.

其他說明

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免責聲明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.