VP303

5092806, human breast, Epithelial

• 分類程式碼代碼: 41106514

屬性

• product name: VP303, 05092806
• 生物源: human breast
• 增長模式: Adherent
• 染色體組型: Mean no. 64, modal no. 67, range 57-72
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Breast Cancer

細胞系描述

Derived from a breast cancer biopsy removed from a 69 year-old female. The histology of the breast cancer biopsy was defined as Ductal Grade II, 90% of tumour cells stained positive for oestrogen receptors and cells were negative for progesterone receptor staining. The VP303 cell line derived from the tumour did not stain positive for oestrogen receptors. The individual was recorded to be alive 5 years after surgery performed to remove the tumour. This cell line grows slowly and is relatively difficult to culture; please read the sub-culture routine information closely.

培養基

MEBM (Clonetics, CC-3151) + MEGM SingleQuots (Clonetics, CC-4136) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS). Fresh EGF (final concentration of 10ng/ml) should be added to the medium at each subculture.

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. The VP series of breast cancer cell lines are very slow growing and relatively difficult to grow. Fresh EGF (final concentration 10ng/ml) s

其他說明

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