COS-7-InVitrus

5063001, African green monkey kidney, Fibroblast

• 分類程式碼代碼: 41106514

屬性

• product name: COS-7-InVitrus, 05063001, from African green monkey kidney, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• 生物源: African green monkey kidney
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 受體: Not specified

描述

細胞系來源

Monkey African green kidney, SV40 transformed, serum-free

細胞系描述

COS-7 cell line (Sigma catalog no. 87021302) adapted to grow in the chemically defined protein- and peptide-free InVitrus medium (Cell Culture Technologies). This cell line is substrate dependent, i.e., adherent. COS-7 was derived from CV-1 simian cells transformed by an origin-defective mutant of SV40.

培養基

InVitrus medium (Cell Culture Technologies http://www.cellculture.com/) + 4 mM L-Glutamine (G7513) + 5 ug/ml Insulin. When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO (D2650).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2, i.e., seeding at 5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Please note: As the cell culture medium does not contain protein it will not inactivate trypsin. Once cells have detached from the flask it is necessary to dilute the trypsin with the cell culture medium, centrifuge the cells and resuspend in fresh medium. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

其他說明

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