L929/R

4102001, mouse adipose tissue, Fibroblast

• 分類程式碼代碼: 41106514

屬性

• 產品名稱: L929/R, 04102001
• 生物源: mouse adipose tissue
• 包裝: tube of 5 μg 04102001-DNA-5UG; pkg of vial of cells 04102001-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse C3H/An connective tissue cisplatin resistant

細胞系描述

This cisplatin-resistant cell line has been developed by exposure of the parent L929 murine fibroblast cell line (Sigma Catalogue number. 85011425) to increasing concentrations of cisplatin in vitro. L929/R cells can be used in the development of novel anti-cancer treatments. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue.

應用

Development of novel anti-cancer treatments

培養基

RPMI 1640 + 2mM L-Glutamine + 10% Foetal Bovine Serum (FBS) + 0.26μM Cisplatin.

例行更新培養

Split sub-confluent cultures (70-80%) 1:5 to 1:50 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 2% CO₂; 37 C.

其他說明

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