Vero-Hektor

03092503, from African green monkey kidney, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

• 分類程式碼代碼: 41106514

屬性

• 生物源: African green monkey kidney
• 增長模式: Adherent
• 染色體組型: Not Specified
• 形態學: Fibroblast-like
• 受體: Not specified

描述

細胞系來源

African green monkey kidney cells, serum-free

細胞系描述

Vero cell line (Sigma catalog no. 84113001) adapted to grow in the chemically defined protein- and peptide-free medium Hektor G (Cell Culture Technologies). This cell line is substrate dependent, i.e., adherent and may be suitable for the replication of viral particles.

培養基

Hektor G medium (Cell Culture Technologies http://www.cellculture.com/) + 4 mM L-Glutamine (G7513). When freezing cells down use Hektor G medium + 4 mM L-Glutamine (G7513) + 10% DMSO (D2650).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm². Use EDTA only (no trypsin); 5% CO₂; 37 °C.

其他說明

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