推薦產品
product name
Cholera Toxin B subunit, ≥95% (SDS-PAGE), lyophilized powder
品質等級
化驗
≥95% (SDS-PAGE)
形狀
lyophilized powder
分子量
~12 kDa
成份
Protein, ~5% Lowry
雜質
≤0.5% Cholera toxin A subunit (SDS-PAGE)
溶解度
H2O: 10 mg/mL
儲存溫度
2-8°C
SMILES 字串
CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F
InChI
1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)
InChI 密鑰
YDXZSNHARVUYNM-UHFFFAOYSA-N
尋找類似的產品？ 前往 產品比較指南
一般說明
Cholera Toxin is a heterohexameric AB5 enterotoxin. Cholera Toxin B subunit is the the B subunit pentamer of cholera toxin produced by Vibrio cholera. It is generally involved in the cell trafficking and signalling machineries.
應用
Cholera Toxin B subunit has been used:
- for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
- in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.
生化／生理作用
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
特點和優勢
This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
包裝
Package size based on protein content
外觀
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.
重構
When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3 and 1 mM sodium EDTA.
分析報告
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.
訊號詞
Warning
危險聲明
危險分類
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
客戶也查看了
R G Zhang et al.
Journal of molecular biology, 251(4), 550-562 (1995-08-25)
Cholera toxin, a heterohexameric AB5 enterotoxin released by Vibrio cholera, induces a profuse secretory diarrhea in susceptible hosts. Choleragenoid, the B subunit pentamer of cholera toxin, directs the enzymatic A subunit to its target by binding the GM1 gangliosides exposed
Lolke De Haan et al.
Molecular membrane biology, 21(2), 77-92 (2004-06-19)
Cholera toxin (Ctx) from Vibrio cholerae and its closely related homologue, heat-labile enterotoxin (Etx) from Escherichia coli have become superb tools for illuminating pathways of cellular trafficking and immune cell function. These bacterial protein toxins should be viewed as conglomerates
Jung Won Youm et al.
FEBS letters, 579(30), 6737-6744 (2005-11-29)
Beta amyloid (Abeta) is believed one of the major pathogens of Alzheimer's disease (AD), and the reduction of Abeta is considered a primary therapeutic target. Immunization with Abeta can reduce Abeta burden and pathological features in transgenic AD model mice.
Denis A Kleymenov et al.
Frontiers in microbiology, 8, 2341-2341 (2017-12-15)
Environmental or city-scale bioaerosol surveillance can provide additional value for biodefense and public health. Efficient bioaerosol monitoring should rely on multiplex systems capable of detecting a wide range of biologically hazardous components potentially present in air (bacteria, viruses, toxins and
Stefan Achtsnicht et al.
PloS one, 14(7), e0219356-e0219356 (2019-07-06)
Cholera is a life-threatening disease caused by the cholera toxin (CT) as produced by some Vibrio cholerae serogroups. In this research we present a method which directly detects the toxin's B subunit (CTB) in drinking water. For this purpose we
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務