C9080

单克隆抗波形蛋白抗体

clone V9, purified immunoglobulin, buffered aqueous solution

• 同義詞: Monoclonal Anti-Vimentin
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 共軛: CY3 conjugate
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: V9, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~58 kDa
• 物種活性: pig, canine, feline, hamster, rabbit, gerbil, monkey, bovine, chicken, human, horse, rat
• 技術: direct immunofluorescence: 1:200 using cultured cells; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human tonsil; immunohistochemistry (frozen sections): suitable using human tonsil
• 同型: IgG1
• UniProt登錄號: P08670
• 運輸包裝: wet ice
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... VIM(7431) ; rat ... Vim(81818)

描述

一般說明

Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vimentin is expressed in breast myoepithelial cells, osteocytes, Langerhans cells of the skin, Schwann cells and astrocytes.

Vimentin is one of the five major groups of intermediate filaments with a molecular mass of 57 kDa. Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments and are components of most eukaryotic cells.

特異性

在免疫组织化学染色中，抗体将波形蛋白定位于成纤维细胞、内皮细胞、淋巴组织和黑色素细胞中。它也在肉瘤、淋巴瘤和黑色素瘤中染色波形蛋白。

免疫原

猪眼晶状体波形蛋白。

應用

Monoclonal Anti-Vimentin-Cy3 antibody produced in mouse has been used in:

• immunofluorescence and confocal microscopy
• cytoskeleton staining
• double labeling experiments
• immunohistochemical
• immunocytochemical localozation

生化／生理作用

Cy3 Monoclonal Anti-Vimentin reacts with normal and pathological tissue of mesenchymally-derived cells, lens tissue and various cultured cells. It localizes vimentin in fibroblasts, lipocytes, endothelial cells, some lymphoid cells, melanocytes, macrophages, and chondrocytes. The antibody stains tumors derived from these cells, including sarcomas, most lymphomas, melanomas and their metastatic lesions. Co-expression of vimentin with keratin was described in most carcinomas of certain sites (e.g., kidney and thyroid) and certain carcinomas of other sites (e.g., breast).

Vimentin was found to be important for stabilizing the architecture of the cytoplasm. It was found, that activated macrophages secrete vimentin into the extracellular space in vitro.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

儲存和穩定性

Store at 2-8 °C. Protect from prolonged exposure to light. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

法律資訊

Cy is distributed under license from Amersham Biosciences Limited.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)