C7492

Monoclonal Anti-Calreticulin antibody produced in mouse

clone TO-11, tissue culture supernatant, buffered aqueous solution

• 同義詞: Calreticulin Antibody Flow Cytometry, Anti-Autoantigen Ro
• 分類程式碼代碼: 12352203
• NACRES: NA.44

屬性

• 生物源: mouse
• 共軛: unconjugated
• 抗體表格: tissue culture supernatant
• 抗體產品種類: primary antibodies
• 無性繁殖: TO-11, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~50 kDa
• 物種活性: human
• 技術: flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 1:50-1:100 using total cell extract of HeLa cells
• 同型: IgG1
• UniProt登錄號: P27797
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CALR(811)

描述

一般說明

Monoclonal Anti-Calreticulin (mouse IgG1 isotype) is derived from the hybridoma TO-11 produced by the fusion of mouse myeloma cells (P3-X63-AG8.653 cells) and splenocytes from BALB/c mice.

Monoclonal Anti-Calreticulin (mouse IgG1 isotype) is derived from the hybridoma TO-11 produced by the fusion of mouse myeloma cells (P3-X63-AG8.653 cells) and splenocytes from BALB/c mice. Calreticulin is a molecular chaperones in endoplasmic reticulum lumen. Calreticulin acts as a lectin-like chaperone binding oligosaccharide residues of newly synthesized N-linked glycoproteins, and misfolded proteins. Calreticulin is a protein binding Ca²⁺ ions. It is encoded by the CALR gene in humans.

應用

Monoclonal Anti-Calreticulin antibody produced in mouse is suitable for the following applications:

• Flow cytometry
• Immunocytochemistry
• Immunohistochemistry
• Indirect ELISA
• Western blotting

生化／生理作用

Calreticulin and Its variants causes differential effects on thrombopoiesis. Somatic mutations of calreticulin (CALR) gene causes thrombocythemia (ET) and primary myelofibrosis (PMF).

It is believed to play a critical role in quality control processes during protein synthesis and folding. Increased expression of calreticulin increases Ca²⁺ storage capacity of the ER. It also appears to modulate store-operated Ca²⁺ -influx, and to alter Ca²⁺ transport by the sarcoplasmic/ER Ca²⁺ -ATPase (SERCA). Overexpression of calreticulin results in increased sensitivity of HeLa cells to drug-induced apoptosis. In contrast, calreticulin-deficient cells show increased resistance to apoptosis. Calreticulin gene disruption leads toimpairment of cardiac development. Loss of calreticulin function favors ubiquitin-proteosome activity. It plays a critical role in Ca²⁺ homeostasis.

外觀

Culture supernatant solution containing 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)