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Merck
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重要文件

C4407

Sigma-Aldrich

Human Collagen Type III

from human placenta, powder, suitable for cell culture

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.32

產品名稱

胶原蛋白 来源于人类胎盘, Bornstein and Traub Type III (Sigma Type X), powder

生物源

human placenta

品質等級

化驗

≥90% (GE)

形狀

powder

技術

cell culture | mammalian: suitable

雜質

HIV, hepatitis B and hepatitis C, none detected

溶解度

aqueous acid: soluble

UniProt登錄號

儲存溫度

2-8°C

基因資訊

human ... COL3A1(1300)

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應用

来自人胎盘的胶原蛋白已用于评估奎宁对气道上皮细胞的生物电效应、研究工程化慢病毒裂解肽的选择性毒性以及用于颗粒聚集测定以快速检测金黄色葡萄球菌( Staphylococcus aureus)上的纤连蛋白、纤维蛋白原和胶原蛋白受体。 在表征人肺肿瘤相关抗原时,它还用于镁依赖型胶原蛋白结合试验。

成分

所有胶原蛋白分子均由以三螺旋构象排列的三条肽链组成,其一级结构主要以三个氨基酸作为重复基序,即每隔两个氨基酸是一个甘氨酸残基,其前面的两个位置通常为脯氨酸或4-羟基脯氨酸。

注意

该产品应在2-8 °C下保存,在此条件下活性可保持2年。

準備報告

它是冻干粉,可以1 mg/mL溶于无菌的.5 M乙酸、PBS或水中。 PBS溶液在-20°C下至少可稳定保存一年。

分析報告

SDS聚丙烯酰胺凝胶电泳在和基底膜胶原蛋白相同的还原条件下测试,得到三条主要条带。

其他說明

胶原蛋白被分为许多种结构和遗传性不同的类型。 我们使用 Bornstein和Traub提出的系统命名法。 小心不要混淆Sigma命名系统和通用的胶原蛋白分类类型。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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