C4237

Protamine Kinase, Cytosolic from bovine kidney

>90% (SDS-PAGE), buffered aqueous glycerol solution

• 同義詞: Cytosolic Protamine Kinase
• MDL號碼: MFCD04041070
• 分類程式碼代碼: 12352204
• NACRES: NA.54

屬性

• 品質等級: 200
• 化驗: >90% (SDS-PAGE)
• 形狀: buffered aqueous glycerol solution
• 比活性: ≥15,000 units/mg protein
• 分子量: 45 kDa
• 包裝: vial of 100 ng
• 運輸包裝: dry ice
• 儲存溫度: −70°C

描述

應用

Cytosolic Protamine Kinase (cPK) is isolated from bovine kidney and has a molecular mass of approximately 45 kDa. It phosphorylates the eukaryotic Initiation Factor 4E (eIF4E), which initiates translation by mRNA. It is used to study protein synthesis and various cellular processes.

生化／生理作用

Cytosolic protamine kinase is involved in the regulation of protein synthesis and is indirectly associated with numerous cellular processes. Cytosolic protamine kinase is a distinct insulin-stimulated kinase involved in the phosphorylation of eukaryotic Initiation Factor 4E (eIF4E) which is key to initiating translation by mRNA. This protein appears to be inactivated by protein phosphatase 2A family members and may also be inhibited by microcystin, okadeic acid, and ATP. The phosphorylation process is reversible and MBPK1 and MBPK2 (Myelin Basic Protein Kinase 1 and 2) may reactivate cytosolic protamine kinase .

單位定義

One unit will incorporate 1 nmol of phosphate using protamine sulfate as a substrate per minute at pH 7.0 at 30 °C.

外觀

Solution in 50 μl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-mercaptoethanol, 1mM benzamidine, 0.1 mM PMSF, 1mM EDTA, 50% glycerol

安全資訊

• 儲存類別代碼: 10 - Combustible liquids