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C0897

Sigma-Aldrich

柠檬酸裂解酶 来源于肺炎克雷伯氏杆菌

lyophilized powder, ≥0.20 unit/mg solid

同義詞:

柠檬酸草酰乙酸裂解酶

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About This Item

CAS號碼:
9012-83-3
酶委員會編號:
4.1.3.6 (BRENDA, IUBMB)
EC號碼:
232-740-7
MDL號碼:
MFCD00130807
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

lyophilized powder

品質等級

200

比活性

≥0.20 unit/mg solid

分子量

>5 kDa

儲存溫度

2-8°C

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應用

从肺炎克雷伯氏菌中分离的柠檬酸裂解酶可用于消化低分子量人乳组分（>5 kDa，简称为 5kF）。

生化／生理作用

柠檬酸裂解酶存在于若干微生物中，用于催化柠檬酸盐裂解的第一步，形成草乙酸和乙酸盐。该酶含有 3 个多肽亚基，α-亚基（转移酶）、β-亚基（酰基裂解酶）和 γ-亚基（酰基-载体蛋白）。

單位定義

在 pH 7.6，25℃ 条件下，一个单位每分钟将 1.0 μmole 柠檬酸盐转化为草乙酸。

外觀

冻干品，含有牛血清白蛋白、蔗糖和 MgSO4

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

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S Bekal-Si Ali et al.
Journal of bacteriology, 181(14), 4411-4416 (1999-07-10)
In this paper, we describe two open reading frames coding for a NAD-dependent malic enzyme (mae) and a putative regulatory protein (clyR) found in the upstream region of citCDEFG of Leuconostoc mesenteroides subsp. cremoris 195. The transcriptional analysis of the
Celia W Goulding et al.
Journal of molecular biology, 365(2), 275-283 (2006-10-27)
Fatty acid biosynthesis is essential for the survival of Mycobacterium tuberculosis and acetyl-coenzyme A (acetyl-CoA) is an essential precursor in this pathway. We have determined the 3-D crystal structure of M. tuberculosis citrate lyase beta-subunit (CitE), which as annotated should
R Singh et al.
Journal of biochemical and biophysical methods, 64(3), 189-199 (2005-09-13)
We demonstrate a facile blue native polyacrylamide gel electrophoresis (BN-PAGE) technique to detect two malate-generating enzymes, namely fumarase (FUM), malate synthase (MS) and four oxaloacetate-forming enzymes, namely pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase (PEPCK), citrate lyase (CL) and aspartate aminotransferase (AST).
M Martín et al.
Journal of bacteriology, 182(14), 3904-3912 (2000-06-27)
In this study we describe the expression pattern of the Leuconostoc paramesenteroides citMCDEFGRP operon in response to the addition of citrate to the growth medium. An 8.8-kb polycistronic transcript, which includes the citMCDEFGRP genes, was identified; its synthesis was dramatically
M Isabel Burguete et al.
Dalton transactions (Cambridge, England : 2003), (36)(36), 4027-4033 (2007-09-11)
A turn-on fluorescent indicator for citric acid (citrate) has been developed, displaying high emission enhancement (+1500%) and low interference by other carboxylates. The sensor is based on the non-emissive copper(II) complex of a fluorescent amino amide, which, upon addition of
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