跳轉至內容
Merck
全部照片(1)

文件

BCK-FC555-50

Sigma-Aldrich

EdU Flow Cytometry Kit 555

sufficient for 50 assays

登入查看組織和合約定價


About This Item

分類程式碼代碼:
12161503
NACRES:
NA.22

螢光

λex 546 nm; λem 579 nm

運輸包裝

wet ice

儲存溫度

2-8°C

尋找類似的產品? 前往 產品比較指南

一般說明

Baseclick EdU Flow Cytometry Kit为直接检测DNA合成提供了替代BrdU和[3H]thymidine检测的出色方法。EdU(5-乙炔基-2'.-脱氧尿苷)是一种胸苷核苷类似物,在活性DNA合成期间掺入到DNA之中。与BrdU检测法不同,EdU Flow Cytometry Assay不依赖抗体,因而不需进行DNA变性来检测掺入的核苷。恰恰相反,EdU Flow Cytometry Kit利用点击化学法来检测不同种类的染料荧光示值。此外,该试剂盒的实验方案经过简化,减少了总步骤数同时大幅缩减了总耗时。简单的点击化学法检测步骤可在30分钟内完成,在结果的内容和情境丰富程度方面,堪比多重分析方法。

應用

流式细胞术

訊號詞

Danger

危險分類

Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Sens. 1

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Shicheng Su et al.
Cell, 175(2), 442-457 (2018-10-06)
Antibody-dependent cellular cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP) critically contribute to the efficacy of anti-tumor therapeutic antibodies. We report here an unexpected finding that macrophages after ADCP inhibit NK cell-mediated ADCC and T cell-mediated cytotoxicity in breast cancers and lymphomas.
Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務