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Merck
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重要文件

BCK-FC488-100

Sigma-Aldrich

EdU流式细胞术试剂盒488

sufficient for 100 assays

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.22

製造商/商標名

(BCK- EdU488FC100)

螢光

λex 496 nm; λem 516 nm

運輸包裝

wet ice

儲存溫度

2-8°C

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相關類別

一般說明

Baseclick品牌的EdU流式细胞分析试剂盒(EdU Flow Cytometry Kit)为直接检测DNA合成提供了替代BrdU和[3H]胸苷检测的出色方法。EdU(5-乙炔基-2′-脱氧尿苷)是一种胸苷类似物,可在DNA合成活跃期掺入到DNA中。与溴脱氧尿苷(BrdU)检测法不同,EdU流式细胞术法不依赖抗体,因此并不需要进行DNA变性以检测掺入的核苷。恰恰相反,EdU流式细胞试剂盒利用点击化学法来检测不同种类的染料荧光示值。此外,简化的检测方案不仅减少总步骤数,而且大幅缩短总用时。这一简单的点击化学检测程序在30分钟内即可完成,兼容的多重检测可提供内容和含义丰富的结果。

應用

流式细胞术

訊號詞

Danger

危險分類

Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Sens. 1

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a

文章

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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