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Merck
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重要文件

B9183

Sigma-Aldrich

单克隆抗-HA

clone HA-7, purified from hybridoma cell culture

同義詞:

抗-HA, 抗-流感血凝素

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.56

生物源

mouse

共軛

biotin conjugate

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

HA-7, monoclonal

形狀

buffered aqueous solution

濃度

~1 mg/mL

技術

western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts.

運輸包裝

dry ice

儲存溫度

−20°C
2-8°C

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一般說明

The Monoclonal Anti-HA Biotin Conjugate antibody recognizes the HA tag sequence on the HA tagged fusion proteins when expressed at the N or C terminal to the fusion protein. This antibody is derived from the HA-7 hybridoma, which is produced from the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH. Ascites fluid collected from the HA-7 hybridoma is then conjugated to biotin.

特異性

该抗体可识别HA-标签蛋白的天然和还原变性形式,并且可与 大肠杆菌 或哺乳动物表达的N-末端或C-末端HA-标签融合蛋白发生反应。

免疫原

与人流感病毒血凝素(HA)片段相对应的合成肽,称为HA-标签,与KLH结合

應用

Antibody suitable for immunoblotting.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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