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Key Documents

B8438

Sigma-Aldrich

阿尔新蓝 溶液

1% in 3% acetic acid, pH 2.5

同義詞:

阿尔新蓝8XG 溶液

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About This Item

分類程式碼代碼:
12171500
NACRES:
NA.47

形狀

liquid

濃度

1% in 3% acetic acid, pH 2.5

顏色

dark blue to very dark blue

pH值範圍

2.4-2.6

Amax

0.45-0.60 (1:400 in H2O)

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

應用

阿尔新蓝溶液已用于对羧化或硫酸化的酸性糖胺聚糖(GAG;粘多糖)进行组织学染色。在pH为2.5时,硫酸糖胺聚糖似乎是阿尔新蓝的首选底物。阿尔新蓝用于染色透明质酸(HA,一种非硫酸化糖胺聚糖),但透明质酸的染色强度受所用组织固定方法的影响。组织中糖胺聚糖的阿尔新蓝染色,可以通过添加诸如氯化镁的电解质来改进。

生化/生理作用

阿尔新蓝是一种阳离子染料。它与酸性糖胺聚糖形成不溶性复合物,从而有助于糖胺聚糖的定量测定。它还用于骨和软骨的染色。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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A procedure for differential staining of cartilage and bone in whole formalin-fixed vertebrates.
Wassersug R J.
Stain Technology, 51(2), 131-134 (1976)
W Lin et al.
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The apparent intensity of hyaluronan (HA) staining in tissue sections can vary as a function of fixation techniques. We examined the histochemical distribution of HA in normal human skin using an HA-specific binding peptide derived from bovine nasal cartilage. The
The quantitative determination of glycosaminoglycans in urine with Alcian Blue 8GX.
Whiteman P.
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