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Key Documents

B6901

Sigma-Aldrich

Monoclonal Anti-Bovine IgG antibody produced in mouse

clone BG-18, ascites fluid

同義詞:

Monoclonal Anti-Bovine IgG

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

ascites fluid

抗體產品種類

secondary antibodies

無性繁殖

BG-18, monoclonal

包含

15 mM sodium azide

物種活性

bovine (non-reactive to IgM)

應無反應活性

pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat

技術

dot blot: suitable
indirect ELISA: 1:1000

同型

IgG1

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

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相關類別

一般說明

The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction.
The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction. IgG (immunoglobulin G) contributes to 10–20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical γ or γ light (L) chains of 50 kDa and 25 kDa, respectively.

特異性

bovine IgG1 and IgG2

免疫原

Purified bovine IgG

應用

Monoclonal Anti-Bovine IgG antibody has been used in indirect immunoperoxidase staining.
Monoclonal Anti-Bovine IgG antibody produced in mouse is suitable for ELISA at a working dilution of 1:20,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. Monoclonal Anti-Bovine IgG antibody produced in mouse was used as an isotype control in ChIP assay.

生化/生理作用

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

外觀

Ascites fluid containing 0.1% sodium azide

儲存和穩定性

For continuous use, store at 2-8°C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Histological and Immunohistochemical Studies on the Furstenberg's Rosette in Cows.
Asti R N, et al.
Kafkas U?niversitesi veteriner faku?ltesi dergisi, 17(2), 520-520 (2011)
Behnaz Heydarchi et al.
mAbs, 9(3), 550-566 (2016-12-21)
We isolated HIV-1 Envelope (Env)-specific memory B cells from a cow that had developed high titer polyclonal immunoglobulin G (IgG) with broad neutralizing activity after a long duration vaccination with HIV-1AD8 Env gp140 trimers. We cloned the bovine IgG matched
Clare F J Grant et al.
Journal of virology, 91(9) (2017-02-24)
Foot-and-mouth disease virus (FMDV) is a highly contagious viral disease. Antibodies are pivotal in providing protection against FMDV infection. Serological protection against one FMDV serotype does not confer interserotype protection. However, some historical data have shown that interserotype protection can
Ulrike I Mödder et al.
The Journal of biological chemistry, 284(28), 18767-18777 (2009-05-09)
Both estrogen receptor (ER) and peroxisome proliferator-activated receptor gamma (PPARgamma) regulate bone metabolism, and because steroid receptor coactivator (SRC)-2 (TIF-2) enhances ER and PPARgamma activity, we examined the consequences of deletion of SRC-2 on bone using SRC-2 knock out (KO)
J Phillips et al.
Cytotherapy, 3(3), 233-242 (2002-08-13)
CAMPATH-1 Abs have been used for T-cell depletion in stem-cell transplantation since the early 1980s. During that time there has been substantial progress in manufacturing techniques and quality control procedures. This article summarizes the methods used to produce the Abs

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