跳轉至內容
Merck
全部照片(1)

Key Documents

View All Documentation

B6149

Sigma-Aldrich

5-溴-4-氯-3-吲哚磷酸 二钠盐

chromogenic, ≥98% (HPLC), powder

同義詞:

BCIP®, X-磷酸盐 二钠盐

登入查看組織和合約定價
全部照片(1)

About This Item

線性公式:
C8H4BrClNO4P · 2Na
CAS號碼:
102185-33-1
分子量：:
370.43
MDL號碼:
MFCD00036757
分類程式碼代碼:
12352204
PubChem物質ID:
24891897
NACRES:
NA.83

product name

5-溴-4-氯-3-吲哚磷酸 二钠盐, ≥98% (HPLC)

品質等級

200

化驗

≥98% (HPLC)

形狀

powder

溶解度

H2O: 20 mg/mL
DMF: insoluble

儲存溫度

−20°C

SMILES 字串

[Na+].[Na+].[O-]P([O-])(=O)Oc1c[nH]c2ccc(Br)c(Cl)c12

InChI

1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2

InChI 密鑰

OAZUOCJOEUNDEK-UHFFFAOYSA-L

尋找類似的產品？ 前往 產品比較指南

相關類別

應用

5-溴-4-氯-3-吲哚磷酸二钠盐已用于检测蛋白-抗体复合物。还用于碱性磷酸酶组化分析的组织染色。
用于碱性磷酸酶标记分子比色检测的有 5-溴-4-氯-3-吲哚磷酸 (BCIP) 和硝基四氮唑蓝 (NBT)。BCIP/NBT 底物系统功能多样，可用于多种应用，包括 Northern、Southern 和 Western 印迹、原位杂交和免疫组化。

BCIP 有两种盐形式：溶于水的二钠盐和溶于二甲基甲酰胺的对甲苯胺形式。这些盐形式可用于制备与 NBT 和反应缓冲液结合形成碱性磷酸酶底物溶液的储备液。该底物系统与碱性磷酸酶共同孵育时，会产生一种不溶性的 NBT 二甲苯胺产物，该产物的紫色易于观察。为方便起见，BCIP 和 NBT 的混合物以易于溶解的片剂形式或即用型液体形式提供。

基底

碱性磷酸酶的组织化学底物。

法律資訊

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

The endosomal protein endotubin is required for enterocyte differentiation
Cox CM, et al.
Cellular and molecular gastroenterology and hepatology, 5(2), 145-156 (2018)
Manli Qi et al.
Journal of bacteriology, 193(10), 2498-2509 (2011-03-29)
The Chlamydia-specific hypothetical protein CT795 was dominantly recognized by human antisera produced during C. trachomatis infection but not by animal antisera raised against dead chlamydia organisms. The immundominant region recognized by the human antibodies was mapped to the N-terminal fragment
Shailesh Kumar et al.
PLoS genetics, 15(6), e1008219-e1008219 (2019-06-27)
Fes1 is a conserved armadillo repeat-containing Hsp70 nucleotide exchange factor important for growth at high temperature, proteasomal protein degradation and prion propagation. Depleting or mutating Fes1 induces a stress response and causes defects in these processes that are ascribed solely
P E Høyer et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 44(8), 855-863 (1996-08-01)
We describe a quantitative histochemical method for demonstration of five N-acetyl-glucosamine binding lectins in the syncytiotrophoblast of human term placenta. The method employs biotinylated lectins and alkaline phosphatase-conjugated avidin. The alkaline phosphatase activity is detected by using 5-bromo-4-chloro-indoxyl phosphate as
Thermal preference ranges correlate with stable signals of universal stress markers in Lake Baikal endemic and Holarctic amphipods
Axenov-Gribanov D, et al
PLoS ONE, 11(10), e0164226-e0164226 (2016)
查看所有相關論文

文章

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務