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重要文件

B4554

Sigma-Aldrich

N,N-双(2-羟乙基)-2-氨基乙磺酸

BioPerformance Certified, suitable for cell culture, ≥99.0%

同義詞:

2-(二乙醇氨基)乙磺酸, N,N-双(2-羟乙基)-2-氨基乙磺酸

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About This Item

經驗公式(希爾表示法):
C6H15NO5S
CAS號碼:
分子量::
213.25
Beilstein:
1781572
EC號碼:
MDL號碼:
分類程式碼代碼:
12161700
PubChem物質ID:
NACRES:
NA.25

等級

BioPerformance Certified

化驗

≥99.0%

形狀

crystalline

技術

cell culture | mammalian: suitable

雜質

endotoxin and bioburden, tested

pH值

2.5-5.0 (25 °C, 213.3 g/L)

有用的pH值範圍

6.4-7.8

pKa (25 °C)

7.1

溶解度

water: 0.5 g/mL, clear, colorless

應用

diagnostic assay manufacturing

異物活動

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

儲存溫度

room temp

SMILES 字串

OCCN(CCO)CCS(O)(=O)=O

InChI

1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)

InChI 密鑰

AJTVSSFTXWNIRG-UHFFFAOYSA-N

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一般說明

N-N-双(2-羟乙基)-2-氨基乙磺酸(BES)是一种两性离子缓冲液,常用于生物化学和分子生物学研究中。BES缓冲液可用作二级pH标准物质,能使溶液保持在稳定的pH范围内,特别是在6.2-7.6范围内。一项研究表明,在细菌内毒素检查中使用这种缓冲液可以最大限度地减少干扰因素的影响。​BES还可用作磷酸钙介导质粒DNA转染真核细胞的缓冲溶液。

應用

BES缓冲液可用于:

  • 作为体外分析胸腺细胞凋亡时培养胸腺细胞用的opti-minimal essential medium(MEM)培养基的补充剂
  • 作为腺相关病毒(AAV)载体制备、提取和纯化用的硼酸盐缓冲液(BBS)的组分
  • 作为精液采集和冷冻保存用的稀释剂之一的组分

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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分析證明 (COA)

Lot/Batch Number

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