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A7510

Sigma-Aldrich

腺苷 5'-三磷酸酶 来源于猪大脑皮质

lyophilized powder, ≥0.3 units/mg protein, pH 7.8

同義詞:

ATP 磷酸水解酶, ATP酶

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About This Item

CAS號碼:
9000-83-3
酶委員會編號:
3.6.1.3 (BRENDA, IUBMB)
MDL號碼:
MFCD00135671
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Porcine cerebral cortex

品質等級

200

描述

Sodium and Potassium-Activated

形狀

lyophilized powder

比活性

≥0.3 units/mg protein, pH 7.8

成份

Protein, 5.0-25.0% biuret

技術

inhibition assay: suitable

UniProt登錄號

P05024
P05027

儲存溫度

−20°C

基因資訊

pig ... ATP1A1(397481) , ATP1B1(396898)

應用

ATP酶用于从ATP中释放无机磷。来自猪大脑皮质的ATP酶已用于Na / K-ATP酶活性的抑制测定。

生化／生理作用

这种膜酶将 ATP 水解为 ADP 和正磷酸盐。水解过程伴随着钠离子和钾离子交换穿过质膜。这种酶几乎存在于高等生物的所有组织中，但在肾脏中含量最多，因为它从肾小球滤液中重吸收钠离子。Na + ,K +-atp 酶受细胞内钙离子变化的影响。它也对哇巴因敏感，并被钒酸盐和（体外）SPAI-1（一种从猪十二指肠分离的肽）抑制。研究表明，它可通过蛋白激酶 A (PKA) 的磷酸化进行调节。蟾酥毒基和其他强心类固醇可能抑制 Na +/K +-atp 酶活性，诱导 HL60、U937 和 ML1 细胞分化。
这种膜酶将 ATP 水解为 ADP 和正磷酸盐。水解过程伴随着钠离子和钾离子交换穿过质膜。这种酶几乎存在于高等生物的所有组织中，但在肾脏中含量最多，因为它从肾小球滤液中重吸收钠离子。Na + ,K +-atp 酶受细胞内钙离子变化的影响。 它也对哇巴因敏感，并被钒酸盐和（体外）SPAI-1（一种从猪十二指肠分离的肽）抑制。研究表明，它可通过蛋白激酶 A (PKA) 的磷酸化进行调节。

單位定義

如果存在Na+, K+和Mg2+，在 pH值为 7.8、37  C 条件下，每分钟从 ATP 释放 1.0 μmol 无机磷

外觀

含有90%蔗糖、0.4%EDTA Na 4+和0.06%NaCl的冻干粉末

準備報告

碘化钠提取部分，Nakao 等人， Biochem. Biophys. Res. Commun., 19, 755 (1965).

抑制劑

產品號碼
描述
訂價

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

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