A5106

Anti-ABI1 兔抗

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 同義詞: Anti-Abelson interactor 1, Anti-Abl-interactor protein 1, Anti-Nap1 binding protein NAP1BP, Anti-eps8 binding protein, Anti-interactor protein AblBP4, Anti-spectrin SH3 domain binding protein 1 SSH3BP1
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~65 kDa
• 物種活性: human, canine (predicted), mouse (predicted), rat
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 濃度: ~1.5 mg/mL
• 技術: western blot: 0.5-1.0 μg/mL using A431 cell lysate; western blot: 0.5-1.0 μg/mL using extract of rat brain cytosolic fraction (S1)
• UniProt登錄號: Q8IZP0
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ABI1(10006) ; mouse ... Abi1(11308) ; rat ... Abi1(79249)

描述

一般說明

Abelson interactor 1 (Abi1) protein, a 65 kD-substrate of the eponymous Abelson non-receptor tyrosine kinase (c-Abl) is expressed mainly in the central nervous system. This gene is located on human chromosome 10p12.

Abi 1 is an adaptor protein that interacts with Wave2 to regulate actin polymerization.

免疫原

synthetic peptide corresponding to amino acids 163-180 of human ABI1, conjugated to KLH. The corresponding sequence is identical in several species including mouse, rat, and dog.

應用

Anti-ABI1 antibody produced in rabbit has been used in western blot analysis and immunofluorescence.

Rabbit anti- ABI1 antibody can be used for immunoblotting assays at a concentration of 0.5-1.0μg/ml using extracts from A431 and rat brain cells.

生化／生理作用

Abelson interactor 1 (Abi1) is essential for the formation and activation of WAVE2 signaling complex. ABI1 and WAVE are essential for Rac-dependent membrane protrusion and macropinocytosis, whereas ABI1 and N-WASP regulate actin-based vesicular transport, EGF receptor endocytosis, and EGFR and transferrin receptor (TfR) cell surface distribution. ABI1 has been shown to act as dual regulator of WAVE and neural Wiskott-Aldrich syndrome protein (N-WASP) specific activities. ABI1 is an essential component of this signaling complex. It positively regulates WAVE activity and connects WAVE to Rac through assembly of the WAVE-ABI1-Nap1-PIR121 complex. Abi1 modulates actin reorganization and lamellipodia formation.

外觀

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)