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一般說明
eAMV™ 逆转录酶是一种增强型禽成髓细胞病病毒（AMV）逆转录酶，负责合成与 RNA、DNA 或 RNA:DNA 杂交体互补的 DNA 链。这种异常强大的 AMV逆转录酶比标准 AMV 或 Moloney 鼠白血病病毒（M-MLV）逆转录酶具有更优的热稳定性。eAMV™ RT 是从总 RNA 或 poly（A）+RNA获得高质量全长 cDNA 的理想酶，并且在转录长靶标方面也是有效的。
應用
Enhanced Avian Reverse Transcriptase可将RNA转录为DNA，同时显著提升mRNA模板合成cDNA的效率。这是由于本款增强型AMV-RT能够逆转录长链mRNA模板，能够有效穿越复杂的二级结构，可以通过RT-PCR反应检测低丰度mRNA。
增强型禽逆转录酶[eAMV™逆转录酶]已用于总 RNA 的逆转录，以在定量逆转录 PCR（RT-qPCR）分析中合成 cDNA。
生化／生理作用
逆转录酶能够加速dNTP形成磷酸二酯键，从而催化RNA模板整合到互补DNA中。
特點和優勢
- 对低丰度信使核糖核酸的敏感性更高
- 在高温（高达 65°C）下通过复杂二级结构进行的无核转录
- 高效生成高达 14.1kb的全长 cDNA
- 产生可用于 PCR 扩增的第一链 cDNA
包裝
提供1管10x反应缓冲液。
單位定義
一单位酶能够以多聚腺苷酸为模板，以12-18的oligo(dT)为引物，在10分钟内将1纳摩TMP整合进TCA沉淀材料中。
法律資訊
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
eAMV is a trademark of Sigma-Aldrich Co. LLC
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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A method of reverse transcription (RT) and polymerase chain reaction (PCR) amplification of 1D (VP1) gene of foot-and-mouth disease (FMD) virus using one reaction mixture containing both avian myeloblastosis virus (AMV) reverse transcriptase (RTase) and Tfl DNA polymerase is described.
Emily Schifano et al.
Virulence, 10(1), 1013-1025 (2019-11-28)
Calcium signaling can elicit different pathways involved in an extreme variety of biological processes. Calcium levels must be tightly regulated in a spatial and temporal manner in order to be efficiently and properly utilized in the host physiology. The Ca2+-ATPase
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We developed a reverse transcription-polymerase chain reaction (RT-PCR) method which permits the simultaneous amplification of several mRNAs, even though their relative levels may be very different. First-strand cDNAs were synthesized from total RNA by MMLV reverse transcriptase with oligo(dT)15 priming.
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British journal of cancer, 91(11), 1970-1976 (2004-11-17)
Interleukin-8/CXCL8 (IL-8) is a chemokine and angiogenic factor. Recently, IL-8 was identified as an autocrine growth factor in several human cancers. Here, we investigated the expression and function of IL-8 in lung cancer cells. The expressions of IL-8 and its
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