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Merck
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重要文件

A2278

Sigma-Aldrich

N-Acetyl-D-glucosamine–Agarose

saline suspension

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About This Item

MDL號碼:
分類程式碼代碼:
41106500
PubChem物質ID:
NACRES:
NA.56

形狀

saline suspension

品質等級

基質

6% beaded agarose

基質活化

divinyl sulfone

基質結合

hydroxyl

基質墊片

5 atoms

容量

≥25 mg/mL binding capacity (Triticum vulgaris lectin)

儲存溫度

2-8°C

SMILES 字串

CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO

InChI

1S/C8H15NO6/c1-4(12)9-5(2-10)7(14)8(15)6(13)3-11/h2,5-8,11,13-15H,3H2,1H3,(H,9,12)/t5-,6+,7+,8+/m0/s1

InChI 密鑰

MBLBDJOUHNCFQT-LXGUWJNJSA-N

一般說明

N-Acetyl-D-glucosamine is used in affinity chromatography, protein chromatography, and carbohydrate matrices. N-Acetyl-D-glucosamine can be used to purify wheat germ (Triticum vulgaris) lectin.

應用

N-Acetyl-D-glucosamine-Agarose has been used

  • for the incubation of supernatant for the depletion of ficolin-3-deficient serum
  • for detecting the lectin activity by ligand binding assays
  • to produce ficolin 2 depleted serum

外觀

Suspension in 0.15 M NaCl, 0.01 M sodium phosphate, pH 6.8, containing 0.02% sodium azide

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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M B Allen et al.
The Biochemical journal, 185(3), 577-582 (1980-03-01)
1. Under normal assay conditions the N-acetyl-D-glucosamine kinases from rat liver and kidney show a pH-dependent lag phase before reaching a steady state, which is probably due to reversible dissociation of the dimeric enzyme. 2. The enzyme catalyses the phosphorylation
Comparative Biochemistry of Molecular Targets for Drug and Pesticides: N-acetyl-D-glucosamine and Acetylcholine Related Enzymes
Yang, Q.Y.
Current Drug Targets (2012)
M B Allen et al.
The Biochemical journal, 185(3), 565-575 (1980-03-01)
1. Procedures for the extensive purification in high yield of N-acetyl-D-glucosamine kinase from rat liver and kidney are described. The separation of this enzyme from hepatic glucokinase depended primarily on their differing behaviour on an affinity column of Sepharose--N-(6-aminohexanoyl)-2-amino-2-deoxy-D-glucopyranose. 2.
J B Pitner et al.
Carbohydrate research, 324(1), 17-29 (2000-03-21)
The binding of Strep 9, a mouse monoclonal antibody (mAb) of the IgG3 subclass directed against the cell-wall polysaccharide of Group A Streptococcus (GAS), has been characterized. The intact antibody and proteolytic fragments of Strep 9 bind differently to GAS:
R Sullivan et al.
Biochemical and biophysical research communications, 162(1), 184-188 (1989-07-14)
Human ejaculated spermatozoa are heterogeneous and can be separated into two distinct populations according to their respective buoyant densities. In order to investigate the functional differences between these two types of spermatozoa, we have searched for the presence of galactosyltransferase.

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