推薦產品
生物源
mouse
重組細胞
expressed in mouse cell line
共軛
agarose conjugate
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
BN-34, monoclonal
形狀
PBS suspension
標籤範圍
2 mg antibody per mL resin
技術
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG1
容量
0.15-0.3 μmol/mL, agarose binding capacity (biotin-ligand conjugate)
應用
research pathology
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
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一般說明
该抗体可使用竞争性ELISA识别游离的生物素和生物胞素。通过使用间接ELISA或免疫组织化学染色，该抗体还可识别与各种免疫球蛋白耦合的生物素。特异性是通过使用生物素化山羊抗体与涂覆在多孔板上的人和兔抗原而评估的。
Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. The use of monoclonal anti-biotin IgG can enhance the sensitivity of such avidin-biotin immunoassays. The monoclonal antibody functions by selectively enlarging and bridging the avidin-biotin-enzyme complexes, thereby increasing the signals from substrate conversion. Furthermore, this antibody can be used in many other applications where biotin may be introduced as target label.
免疫原
生物素-KLH偶联物
應用
该抗体可被用于扩增技术、ELISA、免疫印迹、原位核酸杂交、流式细胞术、图像分析或共聚焦显微镜。
在一些应用中，生物素化探针与亲合素的定位会产生高背景水平。抗生物素试剂可代替亲和素以降低非特异性结合。
在一些应用中，生物素化探针与亲合素的定位会产生高背景水平。抗生物素试剂可代替亲和素以降低非特异性结合。
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Chromatin immunoprecipitation (1 paper)
Monoclonal Anti-Biotin-Agarose antibody produced in mouse has been used in:
- pulldown assay
- coimmunoprecipitation
- chromatin immunoprecipitation
- purification of biotinylated compounds and biotin-containing compounds
Mouse monoclonal anti-biotin antibody was used to identify by western blot S-nitrosylated proteins that had been labeled with a biotin moeity based on the biotin-switch method. No reactivity was visible on the blots when the biotin switch method was inhibited by treatment with 100 mM dithiothreitol showing that this antibody shows no unspecific cross reactivity. The mouse monoclonal anti-biotin antibody does cross react with Arabidopsis plant by western blot, no cross reactivity with unbiotinylated proteins.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
外觀
Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
準備報告
Prepared using purified antibody coupled to cyanogen bromide activated agarose
其他說明
Polyclonal anti-biotin antibodies were shown to react with in vivo biotinylated proteins but not monoclonal anti-biotin antibodies.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Emmanouil Rampakakis et al.
Nucleic acids research, 37(17), 5714-5724 (2009-07-30)
Initiation of DNA replication involves the ordered assembly of the multi-protein pre-replicative complex (pre-RC) during G(1) phase. Previously, DNA topoisomerase II (topo II) was shown to associate with the DNA replication origin located in the lamin B2 gene locus in
A two-step strategy to visually identify molecularly imprinted polymers for tagged proteins
Brandis A, et al.
Journal of Separation Science, 40(16), 3358-3367 (2017)
F J Germino et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(3), 933-937 (1993-02-01)
We describe an in vivo approach for the isolation of proteins interacting with a protein of interest. The protein of interest is "tagged" with a portion of the biotin carboxylase carrier protein (BCCP), encoded on a specially constructed plasmid, so
Transient dsDNA breaks during pre-replication complex assembly
Rampakakis E and Zannis-Hadjopoulos M
Nucleic Acids Research, 37(17), 5714-5724 (2009)
Namrata D Udeshi et al.
Nature methods, 14(12), 1167-1170 (2017-10-19)
Although purification of biotinylated molecules is highly efficient, identifying specific sites of biotinylation remains challenging. We show that anti-biotin antibodies enable unprecedented enrichment of biotinylated peptides from complex peptide mixtures. Live-cell proximity labeling using APEX peroxidase followed by anti-biotin enrichment
相關內容
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
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