79454

Ampliflu Red Westernblot Kit

for fluorescent detection

• 分類程式碼代碼: 12352200

屬性

• 用途: sufficient for 50 applications
• 螢光: λ_ex 571 nm; λ_em 585 nm
• 儲存溫度: 2-8°C

描述

一般說明

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應用

The Ampliflu Red Western Blot Kit provides an alternative to the classical chemiluminescence detection. When working with Ampliflu Red, standard protocols for the immunoblotting-procedure can be used until the detection step. No extra time for signal accumulation is necessary as it is for chemiluminescent signals. The use of Ampliflu Red is suitable for protein quantities between 1 ng and 1 μg per band of protein.

原則

The kit contains the peroxidase substrate Ampliflu Red that is enzymatically converted to the highly fluorescent compound, resorufin. Fluorescent imaging is then performed on the immunoblot.

分析報告

Sensitivity: Limit of detection is 1 ng/band, but may vary depending on the antibodies used.

法律資訊

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

僅套裝組件

• Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC) 3 mL

套裝中的組件也可單獨購買

• 90101 Ampliflu^™ Red, for fluorescence, ≥98.0% (HPLC) 5 vial(s)
• 79382 Phosphate buffered saline, tablet 5 tablets

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H302
• 防範說明: P264 - P270 - P301 + P312 - P501
• 危險分類: Acute Tox. 4 Oral
• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable