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Fluorescent Red Mega 485 NHS-ester

Name: Fluorescent Red Mega 485 NHS-ester
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (coupling to amines)

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About This Item

經驗公式（希爾表示法）:

C₂₉H₃₃N₃O₉S

CAS號碼:

890317-36-9

分子量：:

599.65

MDL號碼:

MFCD06798200

分類程式碼代碼:

12352108

PubChem物質ID:

329760862

NACRES:

NA.32

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產品線

BioReagent

品質等級

100

化驗

≥90% (coupling to amines)

形狀

powder

螢光

λ_ex 482 nm; λ_em 559 nm in 0.1 M phosphate pH 7.0

適合性

suitable for fluorescence

儲存溫度

2-8°C

SMILES 字串

CCN(CCCCCC(=O)ON1C(=O)CCC1=O)c2ccc3C=C(C(=O)Oc3c2)c4cc[n+](CCCS([O-])(=O)=O)cc4

InChI

1S/C29H33N3O9S/c1-2-31(15-5-3-4-7-28(35)41-32-26(33)10-11-27(32)34)23-9-8-22-19-24(29(36)40-25(22)20-23)21-12-16-30(17-13-21)14-6-18-42(37,38)39/h8-9,12-13,16-17,19-20H,2-7,10-11,14-15,18H2,1H3

InChI 密鑰

VKRRBXLGPVGMGA-UHFFFAOYSA-N

應用

Fluorescent Red Mega 485 NHS-ester is an amine conjugating fluorescent biolabel that can be used for covalent coupling to proteins and other biomolecules containing primary amino groups.Fluorescent red Mega 485 and other Mega dyes are specifically designed for multicolor techniques. It is characterized by an extremely large stoke′s shift between excitation and emission maxima. This dye is especially well suited for excitation by argon lasers or other short wavelength excitation light. This means that it can be excited with the same light source or filter arrangement than fluorescein and other widely used dyes as well as other Mega dye. Due to the different emission wavelength several different labels can be detected independently in the same experiment. Due to the small size of the Mega-labels and the direct conversion of excitation light into emission without using a FRET mechanism we foresee a variety of possible applications including DNA sequencing and FISH microscopy.