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Key Documents

51797

Sigma-Aldrich

Atto MB2 maleimide

Methylene Blue derivative, ≥90% (HPLC)

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About This Item

分類程式碼代碼:
12352111
NACRES:
NA.32

化驗

≥90% (HPLC)
≥90% (degree of coupling)

形狀

solid

製造商/商標名

ATTO-TEC GmbH

&lambda ;

in ethanol (with 0.1% trifluoroacetic acid)

紫外吸收

λ: 655-661 nm Amax

儲存溫度

−20°C

應用

ATTO MB2 is a derivative of the well-known redox dye Methylene Blue. The dye can be reversibly reduced to the colorless leuko form. Upon oxidation the dye recovers and the absorption is fully restored. In common with most ATTO-labels the dye shows a high extinction coefficient. The dye is moderately hydrophilic. ATTO MB2 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides react more selectively with thiols. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

法律資訊

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

儲存類別代碼

11 - Combustible Solids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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