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Merck
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重要文件

51449C

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杜氏改良 Eagle 培养基 - 高葡萄糖

with 4500 mg/L dextrose, with 110mg/L sodium pyruvate, without L-glutamine, without phenol red, liquid, sterile-filtered, suitable for cell culture

同義詞:

DME, DMEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

描述

for research or for further manufacturing use

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable
cell culture | stem cell: suitable

成分

phenol red: no
glucose: high
sodium pyruvate: yes
L-glutamine: no

運輸包裝

ambient

儲存溫度

2-8°C

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應用

Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additonally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High". Dulbecco′s Modified Eagle′s Medium/High Modified has been used as a supplement in cell culture.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production
Geors L, et al.
Biotechnology and Bioengineering, 114(6), 1290-1300 (2017)

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