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51146

Sigma-Aldrich

Atto 550 蛋白标记试剂盒

BioReagent, suitable for fluorescence

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

產品線

BioReagent

製造商/商標名

ATTO-TEC GmbH

螢光

λex 544 nm; λem 576 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)

適合性

suitable for fluorescence

儲存溫度

2-8°C

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一般說明

This kit contains sufficient amounts of reactive dye, buffers and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.

應用

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies. Atto 550 Protein Labeling Kit contains Atto 550 succinimidyl ester which enables conjugation of Atto 550 to the primary amino groups of the proteins and other molecules.

法律資訊

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


分析證明 (COA)

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Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
Andrew H J Salmon et al.
Journal of the American Society of Nephrology : JASN, 23(8), 1339-1350 (2012-07-17)
Patients with albuminuria and CKD frequently have vascular dysfunction but the underlying mechanisms remain unclear. Because the endothelial surface layer, a meshwork of surface-bound and loosely adherent glycosaminoglycans and proteoglycans, modulates vascular function, its loss could contribute to both renal
Antibody transfection into neurons as a tool to study disease pathogenesis.
Douglas JN, Gardner LA, et al.
Journal of Visualized Experiments, 26, 4154-4154 (2012)
Sandeep Kumar Vashist
Analytical biochemistry, 421(1), 336-338 (2011-11-19)
A surface plasmon resonance (SPR)-based procedure was developed to determine the effect of antibody modifications on its biomolecular binding behavior. Mouse immunoglobulin G (IgG) was immobilized on a protein A-functionalized gold-coated SPR chip. Goat anti-mouse IgG and its various commercially
Michel Frigoli et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 15(33), 8319-8330 (2009-07-04)
Core-shell type dual fluorescent nanoparticles (NPs) in the 16 nm diameter range with a selective ligand (cyclam) attached to the surface and two fluorophores--9,10-diphenyl-anthracene (donor, D) and pyrromethene PM 567 (acceptor, A)--embedded within the polymer core were synthesized and their

文章

Sigma® offers protein labeling kits based on two types of fluorescent dyes, the Atto dyes and the Tracy dyes.

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