生物源
human
重組細胞
expressed in E. coli
描述
0.1 mg recombinant human E-Cadherin in 20 mM Tris-HCl buffer, containing NaCl, KCl, EDTA, L-arginine, DTT and glycerol.
無菌
Filtered sterilized solution
化驗
≥90% (SDS-PAGE)
形狀
liquid
包裝
pkg of 100 μg
濃度
0.5 mg protein/mL
技術
cell culture | mammalian: suitable
登錄號
NP_004351.1
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... CDH1(999)
應用
上皮钙黏蛋白已作为包被基质蛋白,用于 1)维持长期 ES 或 iPS 细胞培养,然后与适当的 ES 细胞培养基相结合,2)作为包被基质材料,用于极低非特异相互作用的蛋白源 iPS 方案的11R标签重组 TF 细胞内传递,以及 3)作为天然抗原用于抗体生产。
以这些建议作为指导,确定您的培养系统的最佳包被条件。
1. 解冻上皮钙黏蛋白,用无血清培养基或 PBS 稀释至所需浓度。最终溶液应充分稀释,使加入的量均匀覆盖表面。
注:6 孔板中每孔使用 1 mL PBS。
2. 加入 5-10μg 蛋白至各孔,并在2~10°C 下培养一夜。
3. 培养后,抽吸剩余材料。
4. 孔板即时可用。可将其以潮湿状态储存在 2-8°C下或晾干(如果需要保持无菌)。
以这些建议作为指导,确定您的培养系统的最佳包被条件。
1. 解冻上皮钙黏蛋白,用无血清培养基或 PBS 稀释至所需浓度。最终溶液应充分稀释,使加入的量均匀覆盖表面。
注:6 孔板中每孔使用 1 mL PBS。
2. 加入 5-10μg 蛋白至各孔,并在2~10°C 下培养一夜。
3. 培养后,抽吸剩余材料。
4. 孔板即时可用。可将其以潮湿状态储存在 2-8°C下或晾干(如果需要保持无菌)。
序列
MDWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIP
準備報告
用密码子优化法构建重组人上皮钙黏蛋白基因(155-710 aa 断片),并以非融合蛋白形式在 大肠杆菌 中表达为包涵体。使用独特的“温度漂移包涵体复性”技术对最终产物进行复性和色谱纯化。
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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The Wnt/β-catenin signalling is aberrantly activated in primary B cell chronic lymphocytic leukaemia (CLL). Epigenetic silencing of pathway inhibitor genes may be a mechanism for its activation. In this study, we investigated systematically and quantitatively the methylation status of 12
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Oestrogen receptor alpha (ERα) is a well-known target of endocrine therapy for ERα-positive breast cancer. ERα-negative cells, which are enriched during endocrine therapy, are associated with metastatic relapse. Here we determine that loss of ERα in the invasive front and
The Journal of experimental medicine, 210(13), 2939-2950 (2013-12-11)
Type 2 innate lymphoid cells (ILC2s, nuocytes, NHC) require RORA and GATA3 for their development. We show that human ILC2s express skin homing receptors and infiltrate the skin after allergen challenge, where they produce the type 2 cytokines IL-5 and
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Leptospirosis, caused by pathogenic species of Leptospira, is the most widespread zoonosis and has emerged as a major public health problem worldwide. The adhesion of pathogenic Leptospira to host cells, and to extracellular matrix (ECM) components, is likely to be
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