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Key Documents

3023

Sigma-Aldrich

AptaFluor® SAH甲基转移酶测定

同義詞:

Methyltransferase Activity Assay

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About This Item

分類程式碼代碼:
41122100
NACRES:
NA.84

運輸包裝

dry ice

儲存溫度

−70°C

一般說明

AptaFluor® SAH甲基转移酶TR-FRET分析是将S-腺苷甲硫氨酸(SAM)转化为S-腺苷同型半胱氨酸(SAH)的酶的通用分析方法,具有远红色的时间分辨Förster共振能量转移(TR-FRET)读数。该测定法可用于组蛋白甲基转移酶(HMT)和DNA甲基转移酶(DNMT)家族中的所有酶。该测定法专为高通量筛选(HTS)设计,具有简单的混合和读取格式和2个液体添加步骤。它在终点模式下运行:用通用终止试剂(提供)淬灭甲基转移酶(MT)酶反应,然后加入检测试剂。

该测定试剂具有试剂稳定性,并与常用的多模式酶标仪兼容。AptaFluor® SAH甲基转移酶TR-FRET分析具有以下优点:
  • 简单,混合和读取格式非常适合HTS。
  • 在初始速度条件下(=SAM到SAH的20%转换)进行稳健的MT检测。
  • 可容纳低至100 nM和高至5 μM的SAM浓度。
  • 远红色示踪剂进一步减少了荧光化合物和光散射的干扰。

查看完整的Transcreener®产品列表

數量

3023-B=100次测定,96孔
3023-1K=1,000次测定,384孔

外觀

含缓冲水溶液的试剂盒

法律資訊

AptaFluor is a registered trademark of BellBrook Labs
Transcreener is a registered trademark of BellBrook Labs

分析證明 (COA)

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