推薦產品
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
The Transcreener® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener® HTS Assay platform eliminates delays involved in assay development for new HTS targets, and greatly simplifies compound and inhibitor profiling across multiple target families.
The Transcreener® GDP Assay is a far-red, competitive fluorescence polarization (FP) assay based on the detection of GDP and therefore is compatible with any enzyme that produces GDP, including GTPases such as CDC42. The Transcreener® GDP Assay is a simple one step homogenous detection assay, and is extremely flexible with regard to GTP concentration (1 to 1,000 μM GTP). The assay provides excellent signal at low substrate conversion, with a Z′ = 0.7 and =85 polarization shift (mP) at 10% GTP conversion using 10 μM GTP.
The Transcreener® GDP Assay was developed to follow the progress of any enzyme that produces GDP. The Transcreener® GDP Detection Mixture comprises a GDP Alexa633 Tracer bound to a GDP Antibody. The tracer is displaced by GDP, the invariant product generated during the enzyme reaction. The displaced tracer freely rotates leading to a decrease in fluorescence polarization. Therefore, GDP production is proportional to a decrease in polarization. The assay uses a far red tracer to minimize interference from fluorescent compounds and light scattering.
View full Transcreener® product list
The Transcreener® GDP Assay is a far-red, competitive fluorescence polarization (FP) assay based on the detection of GDP and therefore is compatible with any enzyme that produces GDP, including GTPases such as CDC42. The Transcreener® GDP Assay is a simple one step homogenous detection assay, and is extremely flexible with regard to GTP concentration (1 to 1,000 μM GTP). The assay provides excellent signal at low substrate conversion, with a Z′ = 0.7 and =85 polarization shift (mP) at 10% GTP conversion using 10 μM GTP.
The Transcreener® GDP Assay was developed to follow the progress of any enzyme that produces GDP. The Transcreener® GDP Detection Mixture comprises a GDP Alexa633 Tracer bound to a GDP Antibody. The tracer is displaced by GDP, the invariant product generated during the enzyme reaction. The displaced tracer freely rotates leading to a decrease in fluorescence polarization. Therefore, GDP production is proportional to a decrease in polarization. The assay uses a far red tracer to minimize interference from fluorescent compounds and light scattering.
View full Transcreener® product list
數量
3009-1K = 1,000 assay, 384-well
3009-10K = 10,000 assay, 384-well
3009-10K = 10,000 assay, 384-well
外觀
Kit with buffered aqueous solutions
法律資訊
Transcreener is a registered trademark of BellBrook Labs
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務