T15760

Trizma^® 盐酸盐 盐酸盐

reagent grade, ≥99%, crystalline

• 同義詞: TRIS 盐酸盐, 三羟基甲基氨基甲烷 盐酸盐, 三羟甲基氨基甲烷盐酸盐, 盐酸三甲胺
• 線性公式: NH₂C(CH₂OH)₃ · HCl
• CAS號碼: 1185-53-1
• 分子量：: 157.60
• Beilstein: 3675235
• MDL號碼: MFCD00012590
• 分類程式碼代碼: 12161700
• NACRES: NA.25

屬性

• 生物源: synthetic
• 品質等級: 200
• 等級: reagent grade
• 化驗: ≥99%
• 形狀: crystalline
• 儲存條件: (Tightly closed. Dry)
• 雜質: <0.5% water
• 顏色: white
• 有用的pH值範圍: 7.0-9.0
• pKa (25 °C): 8.1
• mp: ~150.7 °C
• 溶解度: water: 1.5 g/mL, clear, colorless
• 正離子痕跡: Fe: ≤5 ppm; Pb: ≤5 ppm
• 儲存溫度: room temp
• SMILES 字串: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI 密鑰: QKNYBSVHEMOAJP-UHFFFAOYSA-N

描述

一般說明

Tris HCl, or Trizma^® hydrochloride, serves as a widely utilized acidic buffer with a pKa of 8.08 at 25°C, offering a pH range of 7.0 to 9.0, aligning with the typical pH of living organisms. It is a crucial component in buffer solutions for diverse biological, cell biology, and biochemistry applications. Notably, Tris base and Tris hydrochloride individually lack sufficient buffering capacity, necessitating their combination to create effective buffers within the pH range of 7 to 9.

Essential in protein electrophoresis and western blotting techniques, Tris buffers, particularly low ionic strength versions, are employed for protein transfer. They are also commonly used in SDS-PAGE gels, running buffers, and blotting buffers. Additionally, Tris salts, integral to DNA agarose electrophoresis, contribute to buffer preparations like TAE (Tris acetate/EDTA) and TBE (Tris borate/EDTA), playing pivotal roles in biochemistry, protein purification, and electrophoresis applications. Overall, Tris HCl proves to be a versatile and indispensable component, ensuring optimal pH in biological systems for effective biochemical and cell biology research.

特點和優勢

• Ideal as a Biological buffer for Cell Biology and Biochemical research
• Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

其他說明

所有缓冲液的pH值都与温度和浓度有关。对于 Tris 缓冲液，温度每降低 1°C，pH 就会增加约 0.03 单位，每稀释 10 倍，pH 会降低 0.03-0.05 单位。对于一些精准的应用，请使用经过仔细校准的pH计和玻璃/甘汞组合电极。

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The pH values of all buffers are temperature and concentration-dependent. For Tris buffers, pH increases by about 0.03 unit per °C decrease in temperature and decreases by 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

法律資訊

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable