SBR00028

FITC labeled vancomycin

for fluorescent microbial imaging

• 同義詞: Vancomycin -FITC, Vancomycin FITC conjugate
• 經驗公式（希爾表示法）: C₈₇H₈₈Cl₂N₁₀O₂₉S
• 分子量：: 1840.65
• 分類程式碼代碼: 51102829
• NACRES: NA.76

屬性

• 品質等級: 100
• 化驗: ≥85% (HPLC)
• 形狀: solid
• 分子量: M_w 1840.66 g/mol
• 存貨情形: not available in China
• 儲存條件: protect from light
• 顏色: yellow to dark orange
• 適合性: suitable for (Fluorescent detection of gram-positive bacteria); suitable for (Microbiome); suitable for microbiology
• 抗生素活性譜: Gram-positive bacteria
• 作用方式: DNA synthesis
• 運輸包裝: dry ice
• 儲存溫度: −20°C

描述

一般說明

FITC labeled Vancomycin (Vancomycin-FITC), is a Fluorescein conjugated Vancomycin, with a similar mode of action as Vancomycin. Vancomycin -FITC can be used to detect Gram-positive bacteria by common fluorescent instruments. Vancomycin is a glycopeptide antibiotic, used to treat gram-positive infections. Fluorescent antibiotics are obtained by synthetic conjugation of an antibiotic to a fluorophore.

應用

Fluorescent antibiotics can be used for many applications including:

• Antimicrobial resistance research.
• Bacterial visualization and imaging.
• Parent antibiotic mode of action research and new antibiotic discovery.
• Toxicity studies.
• Research of bacterial infections and tracking its uptake in vivo.

生化／生理作用

Vancomycin inhibits cell wall synthesis at the level of peptidoglycan biosynthesis. Vancomycin binds with high affinity and specificity to the terminal D-Ala-D-Ala of the growing peptide chain during cell wall synthesis. The binding inhibits transpeptidase activity, which prevents further elongation and cross-linking of the peptidoglycan matrix. Vancomycin-FITC has fluorescein attached to the amine group on the disaccharide moiety of the vancomycin molecule. This disaccharide moiety does not play any role in D-Ala-D-Ala binding site, affording a comparable binding affinity of Vancomycin-FITC to Gram-positive bacteria as the parent drug. However, a minimum inhibitory concentration (MIC) decrease of about two orders of magnitude in comparison to Vancomycin was observed. This activity decrease can be linked to repulsive interactions between the negatively charged Fluorescein moiety and the anionic environment of the peptidoglycan.

特點和優勢

• High-quality antibiotic suitable for multiple research applications
• Ideal for immunological, biochemical, and cell biology applications
• Suitable for fluorescent microscopy imaging

分析報告

• Aliquots of the DMSO solution can be stored at -20°C, protected from light for at least 1 month.
• Fluorescent microscopy application: Vancomycin -FITC has excitation/emission wavelength at 495 nm/515 nm.
• It is recommended to dissolve Vancomycin-FITC in DMSO (Dimethyl Sulfoxide) to a concentration of 1mg/mL.
• Recommended working concentration for Bacillus cereus in fluorescent microscopy imaging application is 25μg/mL.

其他說明

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安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable