PPB015

Tris-Glycine Buffer

pH 8.3, pHast Pack^™, powder

• 同義詞: 1X TG buffer, TG, TG Buffer, Tris Glycine running buffer, Tris Glycine transfer buffer, Tris glycine
• 分類程式碼代碼: 41105319
• NACRES: NA.25

屬性

• 產品線: pHast Pack^™
• 品質等級: 100
• 形狀: powder
• 儲存條件: (Tightly closed. Dry)
• 雜質: ≤5 ppm Heavy Metals (lead)
• 顏色: white to off-white
• pH值: 8.3
• 溶解度: soluble, clear, colorless to faintly yellow
• 正離子痕跡: Fe: <10 ppm
• 異物活動: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• 儲存溫度: room temp

描述

一般說明

Tris-Glycine buffer is primarily used as a running buffer and to prepare transfer buffer in western blot analysis with the addition of methanol. This buffer can separate proteins by their size. It has a pH of 8.3.

應用

Suitable for use as:

• Transfer buffer component in western blot
• Running buffer in native gel electrophoresis for protein separation
• Running buffer in gradient polyacrylamide gel electrophoresis (PAGE)

特點和優勢

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Saves time and minimizes the effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

包裝

Foil pouches

重構

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 25 mM Tris, 192 mM Glycine, pH 8.3 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

儲存和穩定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

其他說明

For additional information on our range of Biochemicals, please complete this form.

法律資訊

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1