推薦產品
等級
certified reference material
品質等級
形狀
liquid
包裝
pkg of 4 x 1 mL
製造商/商標名
NIST®
技術
mass spectrometry (MS): suitable
應用
clinical testing
格式
matrix material
一般說明
测定血清中的总 25-羟基维生素D [25(OH)D]浓度,即25-羟基维生素D2 [25(OH)D2] 和 25-羟基维生素D3 [25(OH)D3]的总和, 一般认为其是维生素D状态的可靠指标。测定血清中的24R, 25(OH)2D3,其被认为是分解代谢谢标志物和肾病的指标。一个SRM 972a 单位含有四个1 mL 冷冻血清样品瓶(水平1到水平4),包含不同浓度水平的25-羟基维生素 D [25(OH)D] 和24R, 25-二羟基维生素D3 [24R,25(OH)2D3].
NIST972A_Cert
NIST972A_SDS
NIST972A_Cert
NIST972A_SDS
應用
冷冻人血清中的维生素D代谢物已被用作参考材料用于通过液相色谱-串联质谱(LC-MS/MS)对干血斑样本中的25-羟基维生素D(25(OH)D)进行校准和标准化 。
该维生素D代谢产物标准参考物质(SRM)主要用作准确的对照品,用于评估人血清中维生素D代谢产物的物质的量浓度的测定方法。 该SRM也用作质量保证工具,用于确定这些成分的内部控制物质的分配值。
生化/生理作用
维生素D,也称为以维生素D3的形式存在的胆钙化固醇。它可通过强化乳制品和鱼油进行膳食摄取。它是由7-脱氢胆固醇通过紫外照射而在皮肤中合成的。维生素D 的生物活性形式,25(OH)2D3,对于矿物质代谢和其他生理功能如抑制癌细胞的生长和防止某些免疫介导的疾病至关重要。
其他說明
- 有关失效日期、储存和使用的信息见于NIST证书。
- 有关生物材料、处置和运输的说明见于NIST MSDS。
準備報告
- 样品瓶应在–20 °C 和 –80 °C之间的温度下在暗处储存。
- 使用前,样品瓶必须在柔和的光线下室温解冻30分钟左右。
法律資訊
NIST is a registered trademark of National Institute of Standards and Technology
SRM is a registered trademark of National Institute of Standards and Technology
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Journal of chromatography. A, 1451, 50-57 (2016-05-18)
A method based on automated on-line solid phase extraction coupled to two-dimensional liquid chromatography with tandem mass spectrometry detection (SPE-2DLC-MS/MS) is here reported for vitamin D metabolite profiling in human serum with absolute quantitation. Two-dimensional LC was configured with two
Clinical chemistry and laboratory medicine, 59(10), 1642-1652 (2021-05-21)
Clinical evaluation of vitamin D status is conventionally performed by measuring serum levels of a single vitamin D metabolite, 25-hydroxyvitamin D predominantly by immunoassay methodology. However, this neglects the complex metabolic pathways involved in vitamin D bioactivity, including two canonical
Overestimation of 25-hydroxyvitamin D3 by increased ionisation efficiency of 3-epi-25-hydroxyvitamin D3 in LC-MS/MS methods not separating both metabolites as determined by an LC-MS/MS method for separate quantification of 25-hydroxyvitamin D3, 3-epi-25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in human serum
Journal of Chromatography. B, Biomedical Sciences and Applications, 967, 195-202 (2014)
Vitamin D: metabolism
Rheumatic Diseases Clinics of North America, 38(1), 1-11 (2012)
Simplified 25-hydroxyvitamin D standardization and optimization in dried blood spots by LC-MS/MS
Journal of AOAC (Association of Official Analytical Chemists) International, 100(5), 1328-1336 (2017)
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