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Key Documents

B4921

Sigma-Aldrich

PhastGel ® 蓝色 R

Pre-measured tablets, readily soluble.

同義詞:

酸性蓝83, Coomassie 亮蓝R, 亮吲哚蓝蛋白 6B, 酸性蓝 83

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About This Item

經驗公式(希爾表示法):
C45H44N3NaO7S2
CAS號碼:
分子量::
825.97
顏色索引編號:
42660
Beilstein:
5718025
EC號碼:
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:

品質等級

SMILES 字串

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI 密鑰

NKLPQNGYXWVELD-UHFFFAOYSA-M

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應用

检测 50-100 ng/条带的大多数蛋白
PhastGel ® Blue R 已用于 SDS-PAGE(十二烷基硫酸钠-聚丙烯酰胺凝胶电泳)后蛋白条带的染色。也用于培养基平板中细胞克隆的染色。
电泳后检测蛋白条带。

重構

1 片制备 400 mL 0.1% 染色液。

法律資訊

PhastGel is a registered trademark of Cytiva

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There
Chun Yi Liau et al.
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A modified Coomassie Brilliant Blue G 250 staining method for detecting chitinolytic enzymes in chitin-containing polyacrylamide gel electrophoresis (PAGE) is presented. The staining formed achromatic zones at the locations of the migrated enzyme. Using Streptomyces griseus chitinase, we have demonstrated

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