89802

4-(二甲氨基)偶氮苯-4′-磺酰氯

for HPLC derivatization, LiChropur^™, 98.0-102.0% (AT)

• 同義詞: 4-(N,N-二甲基氨基)偶氮苯-4'-磺酸氯, 4-二甲胺基苯基偶氮苯磺酰氯, DABS-Cl
• 線性公式: (CH₃)₂NC₆H₄N=NC₆H₄SO₂Cl
• CAS號碼: 56512-49-3
• 分子量：: 323.80
• Beilstein: 3064095
• EC號碼: 260-235-1
• MDL號碼: MFCD00007444
• 分類程式碼代碼: 12352200
• NACRES: NA.22

屬性

• 等級: for HPLC derivatization
• 品質等級: 100
• 化驗: 98.0-102.0% (AT)
• 形狀: crystals
• 品質: LiChropur^™
• 技術: HPLC: suitable
• mp: 185 °C (dec.) (lit.)
• SMILES 字串: CN(C)c1ccc(cc1)\N=N\c2ccc(cc2)S(Cl)(=O)=O
• InChI: 1S/C14H14ClN3O2S/c1-18(2)13-7-3-11(4-8-13)16-17-12-5-9-14(10-6-12)21(15,19)20/h3-10H,1-2H3/b17-16+
• InChI 密鑰: VTVWTPGLLAELLI-WUKNDPDISA-N

描述

一般說明

Amino groups are common to many biological compounds and many amines used in pharmaceuticals. Most of these compounds show weak absorption in direct HPLC analysis with UV-VIS detection. However, strongly absorbing derivatives of amines can be easily detected by HPLC with high sensitivity even when present at low concentrations. Primary and secondary amino groups are usually derivatized as aromatic derivatives by nucleophilic substitution reactions using reagents such as arylsulfonyl chlorides.
4-(Dimethylamino)azobenzene-4′-sulfonyl chloride, a well-known UV-labeling agent, is generally used for the derivatization of N-terminal amino acids, imidazole derivatives, polyamines, etc. prior to their chromatographic determination due to the former′s capacity to covalently bind to the substrates. Its derivatives typically absorb UV radiation between the range of 436-460 nm. This method of derivatization is regarded as superior compared to other methods owing to the simplicity of the procedure, high stability and reproducibility, best resolution of the analytes, etc.

應用

4-(Dimethylamino)azobenzene-4′-sulfonyl chloride may be used to derivatize biogenic amines, primary amino acids, melamine, and hydroxyproline and proline extracted from various biological samples prior to their determination using high performance liquid chromatography (HPLC) technique to increase their detection sensitivity.

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法律資訊

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 象形圖: GHS05
• 訊號詞: Danger
• 危險聲明: H314
• 防範說明: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• 危險分類: Skin Corr. 1B
• 儲存類別代碼: 8A - Combustible corrosive hazardous materials
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable