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Nα-(2,4-二硝基-5-氟苯基)-L氨基丙酰胺

for chiral derivatization, LiChropur, ≥99.0%

同義詞:

FDAA, Marfey 试剂

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About This Item

經驗公式(希爾表示法):
C9H9FN4O5
CAS號碼:
分子量::
272.19
Beilstein:
6820069
MDL號碼:
分類程式碼代碼:
12000000
PubChem物質ID:
NACRES:
NA.22

等級

for chiral derivatization

品質等級

化驗

≥99.0% (sum of enantiomers, TLC)
≥99.0%

形狀

powder

光學活性

[α]20/D +56±2°, c = 1% in acetone

光學純度

enantiomeric ratio: ≥99.5:0.5 (HPLC)

品質

LiChropur

技術

HPLC: suitable

儲存溫度

2-8°C

SMILES 字串

C[C@H](Nc1cc(F)c(cc1[N+]([O-])=O)[N+]([O-])=O)C(N)=O

InChI

1S/C9H9FN4O5/c1-4(9(11)15)12-6-2-5(10)7(13(16)17)3-8(6)14(18)19/h2-4,12H,1H3,(H2,11,15)/t4-/m0/s1

InChI 密鑰

NEPLBHLFDJOJGP-BYPYZUCNSA-N

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一般說明

Nα-(2,4-二硝基-5-氟苯基)-L-丙氨酰胺(FDAA)是一种手性衍生剂(CDA),与其他CDA相比具有高对映选择性和低灵敏度。它常被用于指定痕量氨基酸的立体化学。

應用

在一项使用反相高效液相色谱-电喷雾电离质谱(RPHPLC-ESI-MS)了解罕见氨基酸的研究中,FDAA被用作衍生化试剂。

其他說明

检测非常规手性 α-氨基酸类似物的衍生化试剂

推薦產品

探索最适于 HPLCLC-MS分析的LiChropur试剂

法律資訊

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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Chirality determination of unusual amino acids using precolumn derivatization and liquid chromatography-electrospray ionization mass spectrometry.
Hess S
Journal of Chromatography A, 1035(2), 211-219 (2004)
R Bhushan et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(29), 3148-3161 (2011-07-09)
The present paper describes an updated knowledge and status on Marfey's reagent (MR), 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (FDNP-L-Ala-NH(2)). The reagent is used for pre-column derivatization of amino acids followed by HPLC separation of the diastereomers so formed. Emphasis is put on the
J G Adamson et al.
Analytical biochemistry, 202(1), 210-214 (1992-04-01)
A chromatographic assay has been developed to quantitate racemization occurring during attachment of protected amino acids to peptide synthesis resins. Acidolytic cleavage of deprotected amino acids from supports and subsequent derivatization with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (Marfey's reagent) gave diastereomers separable by
S Kochhar et al.
Analytical biochemistry, 178(1), 17-21 (1989-04-01)
Amino acids are quantitatively determined by precolumn derivatization with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide and reversed-phase high-performance liquid chromatography with photometric detection at 340 nm. Excellent chromatographic resolution of a mixture of the derivatives of 20 amino acids including proline and cystine is
A D Tran et al.
Journal of chromatography, 516(1), 241-249 (1990-09-07)
Separation of amino acid enantiomers and peptide isomers has been made possible through the use of Marfey's reagent and high-performance capillary electrophoresis (HPCE). Samples of amino acids and peptides were first derivatized with Marfey's reagent and subsequently analyzed by HPCE.

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