67651

Y. enterocolitica Millichrome^™ plus Agar Base

suitable for microbiology

• 同義詞: Yersinia chromogenic agar
• 分類程式碼代碼: 41161501
• NACRES: NA.74

屬性

• agency: EP; JP; USP
• 品質等級: 200
• 滅菌相容性: dry heat compatible
• 形狀: dry powder
• 儲存期限: limited shelf life, expiry date on the label
• 成份: Agar, 15.0 g/L ; Chromogenic Mix, 1.3 g/L ; Chromogenic mix, 1.3 g/L ; Peptones, 20.0 g/L ; Salts, 5.0 g/L
• 製造商／商標名: MilliChrome^™
• 技術: microbiological culture: suitable
• 最終pH值: 7.0±0.2 (25.0 °C)
• 溶解度: deionized water: 41.3 g/L
• 應用: food and beverages; microbiology
• 運輸包裝: ambient
• 儲存溫度: 15-25°C
• 適合性: selective for bacteria: Yersinia enterocolitica

描述

一般說明

Y. enterocolitica Millichrome plus Agar Base is a selective media for the detection of Y. enterocolitica. The distinguishing feature of these media is the inclusion of a chromogenic substrate mix that produces visible color changes upon the enzymatic activity of Y. enterocolitica.{TM="Millichrome"] chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The cleavage of the substrate by specific enzymes in the target microorganism releases the chromophore and thus colors the growing microbial colonies. Hence, using chromogenic agars can eliminate the need for subcultures and provide presumptive identification of a chosen microorganism. This approach shortens the turnaround time for microbial diagnosis, reduces the analytical workload, and generates cost savings relative to conventional methods for the isolation and identification of bacterial pathogens

應用

Y. enterocolitica Millichrome plus Agar Base is a selective chromogenic culture medium intended for use in the qualitative direct detection, differentiation, and presumptive identification of pathogenic biotypes of Yersinia enterocolitica from various samples

準備報告

Step 1 (Preparation of 1L Y. enterocolitica Millichrome^™ plus Agar Base)

• Disperse slowly 41.3 g of powder base in 1 L of purified water.
• Stir until agar is well thickened.
• Heat and bring to boil (100 °C) while swirling or stirring regularly. DO NOT HEAT TO MORE THAN 100 °C. DO NOT AUTOCLAVE AT 121 °C.

Warning 1: If using an autoclave, do so without pressure. Advice 1: For the 100 °C heating step, mixture may also be brought to a boil in a microwave oven: after initial boiling, remove from oven, stir gently, then return to oven for short repeated bursts of heating until complete fusion of the agar grains has taken place (large bubbles replacing foam).

• Cool in a water bath to 45-50 °C +/- 2 °C.
• Step 2 (Preparation of the Y. enterocolitica Millichrome^™ plus Supplement)
• Prepare a stock solution of the supplement: Add 100 mg to 1 mL of purified water.
• Swirl well until complete dissolution. Filter sterilize at 0.45 μm.

Warning 2: This supplement stock solution should be used immediately after Preparation, or can be stored at -20 °C and used within 15 days.
Step 3 (Mixing of the prepared base and the prepared supplement)

• Add 1 mL of the prepared supplement solution to the prepared base cooled at 45-50 °C +/- 2 °C.
• Swirl gently to homogenize.
• Pour into sterile Petri dishes.
• Let it solidify and dry.

分析報告

The pathogenic Y. enterocolitica colonies are colored mauve after 48 h of incubation at an optimal growth temperature of 28°C, whereas nonpathogenic strains of the species and occasional isolates of other Enterobacteriaceae grow as metallic blue colonies after as little as 24 h of incubation at the same temperature

法律資訊

MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 危險聲明: H412
• 防範說明: P273 - P501
• 危險分類: Aquatic Chronic 3
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 2