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Merck
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63630

Millipore

D-(+)-蜜二糖

suitable for microbiology, ≥99.0%

同義詞:

6-O-α-D-吡喃半乳糖基-D-葡萄糖

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About This Item

經驗公式(希爾表示法):
C12H22O11
CAS號碼:
分子量::
342.30
Beilstein:
93360
EC號碼:
MDL號碼:
分類程式碼代碼:
41106212
PubChem物質ID:
NACRES:
NA.74

品質等級

化驗

≥99.0% (HPLC)
≥99.0%

形狀

powder

光學活性

[α]20/D +137±3°, 10 hr, c = 5% in H2O

包裝

pkg of 10 g
pkg of 50 g

燃燒殘留物

≤0.1% (as SO4)

顏色

colorless to white

溶解度

H2O: 0.1 g/mL, clear, colorless

負離子痕跡

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤200 mg/kg

正離子痕跡

As: ≤0.1 mg/kg
Ca: ≤500 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤10 mg/kg
K: ≤50 mg/kg
Mg: ≤10 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

應用

microbiology

SMILES 字串

OC[C@H]1O[C@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C12H22O11/c13-1-4(15)7(17)8(18)5(16)3-22-12-11(21)10(20)9(19)6(2-14)23-12/h1,4-12,14-21H,2-3H2/t4-,5+,6+,7+,8+,9-,10-,11+,12-/m0/s1

InChI 密鑰

AYRXSINWFIIFAE-GFRRCQKTSA-N

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一般說明

D-(+)-Melibiose is a reducing disaccharide composed of a unit of galactose linked to a unit of glucose by a glycosidic bond α (1 → 6). It is commonly found in plants and the roots of certain legumes. D-(+)-Melibiose is not metabolized by humans but serves as a substrate for certain bacteria and fungi. It is used as a differential medium component in microbiological media to detect the fermentation of melibiose by microorganisms. It helps identify specific bacterial species based on their ability to ferment melibiose.

應用

D-(+)-Melibiose has applications in the fields of microbiology, research and development, and in food and pharmaceutical industries. Melibiose fermentation test is used to differentiate bacteria like Enterobacteriaceae (like Salmonella, E.coli, shigella, Citrobacter, Klebsiella, Enterobacter) and yeasts. It is also used for the differentiation between the plant pathogenic bacteria Klebsiella pneumoniae and K. variicola.

儲存和穩定性

Keep the container tightly closed in a dry and well-ventilated place.Hygroscopic, Store under inert gas.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

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Saswati Chakladar et al.
Biochemistry, 50(20), 4298-4308 (2011-04-19)
The MelA gene from Citrobacter freundii, which encodes a glycosyl hydrolase family 4 (GH4) α-galactosidase, has been cloned and expressed in Escherichia coli. The recombinant enzyme catalyzes the hydrolysis of phenyl α-galactosides via a redox elimination-addition mechanism involving oxidation of
Jennifer L Chaytor et al.
Glycobiology, 22(1), 123-133 (2011-08-20)
The ice recrystallization inhibition activity of various mono- and disaccharides has been correlated with their ability to cryopreserve human cell lines at various concentrations. Cell viabilities after cryopreservation were compared with control experiments where cells were cryopreserved with dimethylsulfoxide (DMSO).
Lan Guan et al.
Biochemistry, 51(13), 2950-2957 (2012-03-15)
The melibiose permease of Salmonella typhimurium (MelB(St)) catalyzes symport of melibiose with Na(+), Li(+), or H(+), and bioinformatics analysis indicates that a conserved Gly117 (helix IV) is part of the Na(+)-binding site. We mutated Gly117 to Ala, Pro, Trp, or
S Vivek Jakkula et al.
Journal of bacteriology, 194(20), 5538-5544 (2012-08-07)
The melibiose permease of Salmonella enterica serovar Typhimurium (MelB(St)) catalyzes symport of melibiose with Na(+), Li(+), or H(+). Bioinformatics and mutational analyses indicate that a conserved Gly117 (helix IV) is a component of the Na(+)-binding site. In this study, Gly117
Peng Lin et al.
Acta biochimica et biophysica Sinica, 40(12), 1029-1038 (2008-12-18)
A dimeric 50 kDa melibiose-binding lectin was isolated from the seeds of the cultivar of soybean (Glycine max), called the small glossy black soybean. The isolation procedure comprised ion exchange chromatography on Q Sepharose, SP Sepharose and Mono Q followed

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