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Merck
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Key Documents

48300

Millipore

胆汁七叶苷琼脂

suitable for microbiology, NutriSelect® Plus

同義詞:

B.B.E. 琼脂, 七叶苷胆汁琼脂, 类杆菌胆汁七叶苷琼脂

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About This Item

分類程式碼代碼:
41171606
NACRES:
NA.74

agency

according to ISO 10273:2017

品質等級

無菌

non-sterile

產品線

BioChemika

形狀

powder

儲存期限

limited shelf life, expiry date on the label

成份

agar, 15.0 g/L
esculin, 1 g/L
ferric citrate, 0.5 g/L
meat extract, 3 g/L
meat peptone, 5 g/L
ox-bile, 40 g/L

製造商/商標名

NutriSelect® Plus

技術

microbe id | specific enzyme detection: suitable

最終pH值

6.6±0.2 (25 °C)

應用

environmental
food and beverages
water monitoring

microbiology

適合性

Streptococcus spp.
selective and differential for Enterococcus spp.

應用

Bile esculin agar (BEA) is a differential and selective medium and is mainly used to discern group D Streptococci and Enterococci based on the organism′s potential to hydrolyze esculin. BEA comprises of oxygall (bile salts, first selective ingredients) and azide (second selective ingredients), former inhibits the growth of gram positive organisms and latter inhibits the growth of gram negative organisms. BEA also comprises of nutrients ferric citrate and esculin, where esculin is a differential ingredient and is also a fluorescent compound, upon hydrolyzation the fluorescence is lost. BEA is used for bile esculin test which is based on the hydrolysis of esculin into esculetin (6, 7-dihydroxy-coumarin) and glucose by a micro-organism that produce an enzyme esculinase.
For the preliminary identification of enterococci (serology group D), as defined by Swan (1954) and Facklam and Moody (1970).

準備報告

Dissolve 64.5 g in 1 litre distilled water. Bring to a boil while stirring continually. This medium should NOT be autoclaved. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes.

腳註

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法律資訊

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

Lot/Batch Number

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Bile Esculin Test- Principle, Procedure, Result Interpretation and Limitation. Microbiology notes. Pratiksha Pokhrel September 1, 2015 Bile Esculin Test- Principle, Procedure, Result Interpretation and Limitation2015-10-16T17:40:41+00:00 Bacteriology, Biochemical Test, Diagnosis, Laboratory.
Science (2015)
Use of bile-esculin agar for rapid differentiation of Enterobacteriaceae.
Lindell, SHIRLEY S., and P. A. T. R. I. C. I. A. Quinn
Journal of Clinical Microbiology, 1 (5), 440-443 (1975)
R R Facklam et al.
Applied microbiology, 20(2), 245-250 (1970-08-01)
Six tests commonly used for the presumptive identification of group D streptococci were evaluated. Strains tested included 282 group D streptococci and 366 non-group D. Ratios of percentages of group D to non-group D strains which gave positive reactions for
The use of a bile-aesculin medium and of Maxted's technique of Lancefield grouping in the identification of enterococci (group D streptococci).
A SWAN
Journal of clinical pathology, 7(2), 160-163 (1954-05-01)

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