推薦產品
生物源
human serum
品質等級
形狀
lyophilized
比活性
~11 units/mg protein (activated with streptokinase at 37 °C and pH 7.4 with Chromozym PL as the substrate.)
分子量
90-94 kDa
包裝
pkg of 20 U
製造商／商標名
Roche
技術
activity assay: suitable
應用
life science and biopharma
運輸包裝
wet ice
一般說明
Plasminogen is a profibrinolysin from human serum. Plasminogen, the proenzyme of the fibrinolytically active enzyme plasmin, is a single chain glycoprotein. Various isoelectric forms exist and can be separated by means of isoelectric focusing (IEF). The lyophilizate is stabilized with bovine serum albumin (BSA).
應用
Plasminogen is used for the determination of the activity of plasminogen activators. The enzyme is a substrate for tissue plasminogen activator (t-PA) in the t-PA test and a reference substance for t-PA therapy. It is used as the in-process control in the production of t-PA.
特點和優勢
Contents
Lyophilizate, stabilized with bovine serum albumin.
Lyophilizate, stabilized with bovine serum albumin.
品質
Contaminants: <0.05% plasmin; the preparation does not contain 6-aminohexanoic acid
序列
Native plasminogen (glu-plasminogen) has a N-terminal glutamic acid group. Partial plasma proteolysis gives rise to a molecule with N-terminal lysine (lys-plasminogen). Cleavage with elastase results in val-plasminogen. The complete amino acid sequence contains 790 amino acids. Roche Applied Science supplies glu-plasminogen.
準備報告
Stabilizers: stabilized with BSA
Working solution: Solvent is recommended in 20 mM sodium phosphate buffer, pH 7.5, 100 mM NaCl.
Storage conditions (working solution): -15 to -25 °C
A solution in 20 mM sodium phosphate buffer, pH ca. 7.5, 100 mM NaCl is stable for several months without loss of activity when stored at -15 to -25 °C.
Note:
Plasminogen is susceptible to autolysis, which may result in activation. Contact with the skin or other contamination (e.g., by microorganisms), is therefore to be avoided.
Working solution: Solvent is recommended in 20 mM sodium phosphate buffer, pH 7.5, 100 mM NaCl.
Storage conditions (working solution): -15 to -25 °C
A solution in 20 mM sodium phosphate buffer, pH ca. 7.5, 100 mM NaCl is stable for several months without loss of activity when stored at -15 to -25 °C.
Note:
Plasminogen is susceptible to autolysis, which may result in activation. Contact with the skin or other contamination (e.g., by microorganisms), is therefore to be avoided.
儲存和穩定性
Store at 2 to 8 °C. (Store dry!)
其他說明
仅用于生命科学研究。不可用于诊断。
訊號詞
Warning
危險聲明
危險分類
STOT RE 2
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
does not flash
閃點（°C）
does not flash
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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Andrea Schweinitz et al.
The Journal of biological chemistry, 279(32), 33613-33622 (2004-05-20)
The serine protease urokinase-type plasminogen activator (uPA) interacts with a specific receptor (uPAR) on the surface of various cell types, including tumor cells, and plays a crucial role in pericellular proteolysis. High levels of uPA and uPAR often correlate with
Claudine Ferland et al.
Journal of immunology (Baltimore, Md. : 1950), 173(7), 4417-4424 (2004-09-24)
Increased eosinophil counts are a major feature of asthmatic airways. Eosinophil recruitment requires migration through epithelium and tissue extracellular matrix by activation of proteases. We assessed the capacity of IL-16, a CD4(+) cell chemotactic factor, to induce migration of eosinophils
H Roger Lijnen
Biochemistry. Biokhimiia, 67(1), 92-98 (2002-02-14)
Several molecular interactions between the matrix metalloproteinase (MMP) and the plasminogen/plasmin (fibrinolytic) system may affect cellular fibrinolysis. MMP-3 (stromelysin-1) specifically hydrolyzes urokinase (u-PA), yielding a 17 kD NH2-terminal fragment containing the functionally intact receptor (u-PAR)-binding sequence and a 32 kD
A Krüger et al.
Cancer gene therapy, 7(2), 292-299 (2000-04-19)
The serine protease urokinase-type plasminogen activator, uPA, when bound to its specific receptor, uPAR (CD87), plays a significant role in tumor cell invasion and metastasis. In breast cancer, enhanced uPA antigen in the primary tumor is correlated with poor prognosis
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