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一般說明
该引物由15聚体寡核苷酸组成,该寡核苷酸在每个位置均有胸苷(T)。 该寡核苷酸通过 HPLC 纯化、脱盐,并以锂盐冻干粉的形式提供。 p(dT)15 引物的 5′ 末端没有进行磷酸化。Oligo(dT)N 生成全长cDNA。如果模板含有在内部拉伸的oligo(A),则引物可能会与之结合,从而导致引导错误(mis priming)和非全长的转录本。
组成: p(dT)15[ 15聚体寡核苷酸,只含脱氧胸苷(dT)残基].
磷酸化: p(dT)15引物的 5′ 末端没有进行磷酸化。
组成: p(dT)15[ 15聚体寡核苷酸,只含脱氧胸苷(dT)残基].
磷酸化: p(dT)15引物的 5′ 末端没有进行磷酸化。
應用
cDNA合成引物可用于:
- RT-PCR
- cDNA文库的生成
- 第一链cDNA的合成
單位定義
8 nmol ξ 1 A260 unit
其他說明
仅用于生命科学研究。不可用于诊断。
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
nwg
閃點(°F)
does not flash
閃點(°C)
does not flash
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