推薦產品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
4C1.3, monoclonal
形狀
liquid
不包含
preservative
物種活性
mouse, human, rat
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
同型
IgG
儲存溫度
−70°C
目標翻譯後修改
unmodified
基因資訊
human ... PPARGC1A(10891)
一般說明
PGC-1α是PPARα、PPARγ和其他转录因子的共激活因子，可调节棕色脂肪组织中适应性生热、肝/肾糖异生和肌肉纤维类型转换的转录程序。全长蛋白质为113 kDa，通过冷暴露在棕色脂肪组织中诱导，通过禁食在肝脏和肾脏中诱导，并通过运动在骨骼肌中诱导。全长基因的可变剪接产生270aa的N末端剪接变体，其在〜38kDa处迁移并由诱导全长蛋白质表达的相同生理信号诱导。N末端PGC-1α剪接变体也受到翻译后修饰的影响，从而改变其迁移和表观分子量。
蛋白G纯化的小鼠单克隆抗体。识别内源形式的PGC-1α，包括~113 kDa PGC-1α蛋白和~38 kDa剪接变体。
识别棕色脂肪组织、肝脏和肾脏中的内源形式的PGC-1α，包括~113 kDa PGC-1α蛋白和~38 kDa剪接变体。
该抗PGC-1α小鼠单克隆抗体（4C1.3）经验证可用于免疫印迹、免疫细胞化学、免疫沉淀、石蜡切片以检测PGC-1α。
免疫原
小鼠
由小鼠PGC-1的1-120位氨基酸组成的重组蛋白α
應用
免疫印迹（1 µg/mL）
免疫细胞化学（5-10 µg/mL）
免疫沉淀（10 µg/mL）
石蜡切片（整个安装）（请参阅应用参考文献）
免疫细胞化学（5-10 µg/mL）
免疫沉淀（10 µg/mL）
石蜡切片（整个安装）（请参阅应用参考文献）
警告
毒性：多个毒性值，请参阅MSDS（O）
外觀
1小瓶100 µg抗体溶于50 mM PBS中，参见批次特定浓度的小瓶。一小瓶NT-PGC-1α阳性对照，在含有10 mM &-巯基乙醇和1%SDS的RIPA缓冲液中以25 µl全细胞提取物的形式提供；每个泳道上样10 µl；在SDS-PAGE上样前加入样品缓冲液。
重構
初次解冻后，分装冻存（-70°C）。
其他說明
Lai, L., et al. 2008.Genes Dev.14, 1948.
Rodgers, J.T., et al. 2008.FEBS Lett.582, 46.
Mazzucotelli, A., et al. 2007.Diabetes 10, 2467.
Nemoto, S., et al. 2005.J. Biol. Chem. 16, 16456.
Rodgers, J.T., et al. 2008.FEBS Lett.582, 46.
Mazzucotelli, A., et al. 2007.Diabetes 10, 2467.
Nemoto, S., et al. 2005.J. Biol. Chem. 16, 16456.
内源性PGC-1α主要存在于细胞核中，NT-PGC-1α存在于胞质和核部分中。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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Xingyuan Wen et al.
BioMed research international, 2014, 402175-402175 (2014-08-20)
PGC-1α is an inducible transcriptional coactivator that regulates mitochondrial biogenesis and cellular energy metabolism in skeletal muscle. Recent studies have identified two additional PGC-1α transcripts that are derived from an alternative exon 1 (exon 1b) and induced by exercise. Given
Dean-Chuan Wang et al.
International journal of molecular sciences, 21(11) (2020-06-04)
Early-life exposure to di-(2-ethylhexyl)-phthalate (DEHP) has been suggested to relate to hyperactivity, lack of attention, and working memory deficits in school-age children. Brain-derived neurotrophic factor (BDNF) and endocannabinoids are induced by aerobic exercises to provide beneficial effects on brain functions.
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