推薦產品
技術
cell based assay: suitable
檢測方法
fluorometric
運輸包裝
ambient
相關類別
一般說明
Caspases are a family of protease enzymes that play essential roles in programmed cell death, termed apoptosis. Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9 and sequential activation of plays a central role in the execution-phase of cellular apoptosis.
The BioTracker NucView 530 Red Caspase-3 Dye provides a convenient live cell tool for profiling apoptotic cell populations based on caspase-3/7 activity using either fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, the BioTracker NucView 530 Red Caspase-3 Dye can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright red fluorescence. Thus, NucView 530 Caspase-3 Substrate is bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The dye is formaldehyde-fixable and compatible with subsequent immunostaining. The dye is offered in DMSO and PBS (phosphate-buffered saline) formulations. The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity.
NucView 530 Caspase-3 Substrate stains apoptotic cell nuclei with orange fluorescence, for detection in the Cy3 channel by fluorescence microscopy or the PE channel by flow cytometry. Note that when excited by the 488nm laser line, NucView 530 also fluoresces in the FITC channel, and therefore cannot be analyzed together with green probes by flow cytometry.
Spectral Properties
Absorbance: 528nm
Emission: 563nm
The BioTracker NucView 530 Red Caspase-3 Dye provides a convenient live cell tool for profiling apoptotic cell populations based on caspase-3/7 activity using either fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, the BioTracker NucView 530 Red Caspase-3 Dye can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright red fluorescence. Thus, NucView 530 Caspase-3 Substrate is bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The dye is formaldehyde-fixable and compatible with subsequent immunostaining. The dye is offered in DMSO and PBS (phosphate-buffered saline) formulations. The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity.
NucView 530 Caspase-3 Substrate stains apoptotic cell nuclei with orange fluorescence, for detection in the Cy3 channel by fluorescence microscopy or the PE channel by flow cytometry. Note that when excited by the 488nm laser line, NucView 530 also fluoresces in the FITC channel, and therefore cannot be analyzed together with green probes by flow cytometry.
Spectral Properties
Absorbance: 528nm
Emission: 563nm
應用
Live cell fluorescent imaging
Live cell imaging apoptosis dye for caspase-3/7 enzyme activity used to detect programmed cell death using fluorescence microscopy or flow cytometry applications.
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
品質
Spectral Properties
Absorbance: 528nm
Emission: 563nm
Absorbance: 528nm
Emission: 563nm
外觀
Liquid
儲存和穩定性
Store BioTracker NucView 530 Red Caspase-3 Dye, 1mM in DMSO at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
法律資訊
NucView is a registered trademark of Biotium Inc.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
188.6 °F - (refers to pure substance)
閃點（°C）
87 °C - (refers to pure substance)
分析證明 (COA)
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