SCR078
Fluorescent Human ES/iPS Cell Characterization Kit
This Fluorescent Human ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of human Embryonic stem & induced pluripotent Stem cells.
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About This Item
分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.84
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一般說明
Human embryonic stem (hES) cells are pluripotent cells derived from the inner cell mass of pre-implantation blastocysts. Human induced pluripotent (hiPS) cells are pluripotent cells generated by reprogramming human somatic cells using four transcription factors, Oct-4, Klf-4, Sox-2, and c-Myc, or their variants. Both hESC and hiPSC can self-renew and have the ability to generate all three germ layers: ectoderm, mesoderm, and endoderm. In vitro, hESC/iPSC are normally maintained and propagated on mouse fibroblast feeders for extended periods in media containing basic fibroblast growth factor (bFGF). However, spontaneous differentiation may occur in subpopulations of cells. Several pluripotent markers are commonly used to distinguish pluripotent hESC/iPSC from differentiated cells.
EMD Millipore’s Fluorescent Human ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of human ES/iPS cells. Included in the kit is an enzymatic assay to measure alkaline phosphatase activity in the cells along with validated directly conjugated antibodies to pluripotent transcription factors, Oct-4, Sox-2 and Nanog and cell surface epitopes TRA-1-60 and TRA-1-81 to enable rapid immunocytochemical marker analysis. The Dapi nuclear dye is conveniently included to aid in cell quantification. While the expression levels of pluripotent markers are expected to be diminished upon differentiation, each possess specific expression kinetics. For example, it has been noted that upon differentiation, Oct-4 and TRA-1-60 expressions are the first to be down-regulated while Nanog and alkaline phosphatase down-regulate at a much slower timeframe.
EMD Millipore’s Fluorescent Human ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of human ES/iPS cells. Included in the kit is an enzymatic assay to measure alkaline phosphatase activity in the cells along with validated directly conjugated antibodies to pluripotent transcription factors, Oct-4, Sox-2 and Nanog and cell surface epitopes TRA-1-60 and TRA-1-81 to enable rapid immunocytochemical marker analysis. The Dapi nuclear dye is conveniently included to aid in cell quantification. While the expression levels of pluripotent markers are expected to be diminished upon differentiation, each possess specific expression kinetics. For example, it has been noted that upon differentiation, Oct-4 and TRA-1-60 expressions are the first to be down-regulated while Nanog and alkaline phosphatase down-regulate at a much slower timeframe.
特異性
Cross Reactivty
None
None
應用
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
This Fluorescent Human ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of human Embryonic stem & induced pluripotent Stem cells.
成分
1. Fast Red Violet solution (Part No. 90239): One 15 mL bottle.
2. Napthol AS-BI phosphate solution (Part No. 90234). One 15 mL bottle.
3. Mouse anti-Oct-4 (POU5f1), clone 7F9.2, Alexa Fluor™ 488 conjugate (Part No. MAB4419A4-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
4. Mouse anti-Sox-2, clone 10H9.1, Cy3 conjugate (Part No. MAB4423C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
5. Mouse anti-Nanog, clone 7F7.1, Alexa Fluor 488 conjugate (Part No. MABD24A4-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
6. Mouse anti-TRA-1-60, clone TRA-1-60, Cy3 conjugate (Part No. MAB4360C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
7. Mouse anti-TRA-1-81, clone TRA-1-81, Cy3 conjugate (Part No. MAB4381C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
8. DAPI, 100 µL (Part No. 90229). One vial containing 100 µL volume.
2. Napthol AS-BI phosphate solution (Part No. 90234). One 15 mL bottle.
3. Mouse anti-Oct-4 (POU5f1), clone 7F9.2, Alexa Fluor™ 488 conjugate (Part No. MAB4419A4-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
4. Mouse anti-Sox-2, clone 10H9.1, Cy3 conjugate (Part No. MAB4423C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
5. Mouse anti-Nanog, clone 7F7.1, Alexa Fluor 488 conjugate (Part No. MABD24A4-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
6. Mouse anti-TRA-1-60, clone TRA-1-60, Cy3 conjugate (Part No. MAB4360C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
7. Mouse anti-TRA-1-81, clone TRA-1-81, Cy3 conjugate (Part No. MAB4381C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
8. DAPI, 100 µL (Part No. 90229). One vial containing 100 µL volume.
品質
Conjugated antibodies and alkaline phosphatase reagents have been validated on H9 human ES and STEMCCA derived human ips cells.
外觀
Purified conjugated mouse monoclonal antibodies are stored in PBS with 0.1% sodium azide and 15mg/ml BSA.
儲存和穩定性
Store at 2-8°C until expiration date of kit. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
法律資訊
ALEXA FLUOR is a trademark of Life Technologies
訊號詞
Warning
危險聲明
危險分類
Carc. 2 - Eye Irrit. 2 - Skin Irrit. 2
儲存類別代碼
10 - Combustible liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Melissa Conti Mazza et al.
Stem cell research, 55, 102506-102506 (2021-08-23)
Mutations in the oncogene PARK7, which codes for DJ-1, have been associated with early-onset autosomal recessive Parkinson's disease (PD); however, the exact role of DJ-1 in PD remains elusive. Fibroblasts from a PD patient with a uniparental disomy, 1 bp deletion
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