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Key Documents

SCC109

Sigma-Aldrich

AC16 人心肌细胞细胞系

Human

同義詞:

AC16 Ventricular Cardiomyocyte Cell Line

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About This Item

分類程式碼代碼:
41106514
eCl@ss:
32011203
NACRES:
NA.81

product name

AC16 人心肌细胞细胞系, AC16 Human Cardiomyocytes can be serially passaged and can differentiate when cultured in mitogen-free medium. The cells may be used to study developmental regulation of cardiomyocytes.

生物源

human

品質等級

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

一般說明

AC16人心肌细胞系是一种增殖细胞系,源自成人心室心脏组织的原代细胞与SV40转化的尿苷营养缺陷型人成纤维细胞融合,不含线粒体DNA。 在无尿苷培养基中筛选去除未融合的成纤维细胞后,将融合细胞进一步亚克隆,然后筛选是否存在SV40大T-ag,β-肌球蛋白重链(βMHC)和连接蛋白43(CX-43)

通过改变培养条件和沉默SV40 T-ag的表达可以控制AC16细胞的增殖和分化。 这些细胞可用于在正常发育过程中,并且在细胞、细胞器和分子水平的病理条件下研究心脏基因的表达和功能。

細胞系描述

心肌细胞

應用

研究类别
心脏

心血管疾病

毒性
该产品可出售给学术机构或非营利性实体以供研究使用。有关商业许可的信息,请联系licensing@emdmillipore.com。

品質

• 每小瓶含有≥ 1X106个活细胞。
•通过PCR检测细胞,对HPV-16、HPV-18、甲型、乙型、丙型肝炎和HIV-1 & 2型病毒呈阴性。
•细胞对支原体污染呈阴性。
•通过STR分析对每批细胞进行基因分型,以验证细胞系的唯一身份。

儲存和穩定性

AC16人心肌细胞株应保存在液氮中。在初始解冻后,细胞可以培养至少10代,而不会显著影响细胞标志物的表达和功能。

免責聲明

仅供研究使用。在法国,在用于科研目的时(包括进口和出口活动(《公共健康法》第L 1211-1条,第2节))该产品为管制品。购买者(即最终用户)需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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存取文件庫

Xiangyang Wu et al.
Experimental and therapeutic medicine, 18(4), 3172-3176 (2019-10-02)
MicroRNA-663 (miRNA-663) regulates the expression of transforming growth factor β1 (TGF-β1), which participates in the pathogenesis of myocardial fibrosis. Therefore, microRNA-663 may also serve a role in myocardial fibrosis. The present study aimed to determine whether miRNA-663 participates in myocardial
Matthew Alonzo et al.
Progress in biomaterials, 9(3), 125-137 (2020-09-27)
Hydrogels are a class of biomaterials used for a wide range of biomedical applications, including as a three-dimensional (3D) scaffold for cell culture that mimics the extracellular matrix (ECM) of native tissues. To understand the role of the ECM in
Xinyong Cai et al.
Molecular medicine reports, 22(6), 4579-4588 (2020-11-12)
FGD5 antisense RNA 1 (FGD5‑AS1) is a long non‑coding RNA in acute myocardial infarction (AMI), which is primarily caused by myocardial ischemia‑hypoxia. Retinoid acid receptor‑related orphan receptor α (RORA) is a key protector in maintaining heart function. However, the roles of FGD5‑AS1 and RORA
P Litzkas et al.
Molecular and cellular biology, 4(11), 2549-2552 (1984-11-01)
A method for fusion of protoplasts bearing amplified plasmids and human diploid fibroblasts or other cell types in suspension is described. Transient expression of plasmid-encoded proteins occurs in up to 50% of the human cells, as demonstrated for simian virus
Vikram Thakur et al.
Biomolecules, 11(4) (2021-05-01)
Myocardial fibrosis is one of the major complications of long-term diabetes. Hyperglycemia induced cardiomyocyte atrophy is a frequent pathophysiological indicator of diabetic heart. The objective of this study was to investigate the cardioprotective effect of glycyrrhizin (GLC) on myocardial damage

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