RADPCMAG-82K

MILLIPLEX^® Rat Adipocyte Panel - Metabolism Assay

• 分類程式碼代碼: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

屬性

• 物種活性: rat
• 品質等級: 200
• 製造商／商標名: Milliplex^®
• assay range: accuracy: 94-121%; sensitivity: 0.1-25 pg/mL; (MinDC+2SD); standard curve range: 0.7-3,000 pg/mL; (IL-1β, TNFα); standard curve range: 12-50,000 pg/mL; (PAI-1); standard curve range: 122-500,000 pg/mL; (Adiponectin); standard curve range: 15-60,000 pg/mL; (IL-6, Leptin); standard curve range: 9.8-40,000 pg/mL; (MCP-1)
• 技術: multiplexing: suitable
• 檢測方法: fluorometric (Luminex xMAP)
• 運輸包裝: wet ice

描述

一般說明

In the past, the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these Adipokines, Adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body′s response to insulin. Also, its anti-inflammatory effects on the cellular lining of blood vessel walls may help explain the association of high Adiponectin levels with the reduced risk of heart attack.

The MILLIPLEX^® Rat Adipocyte Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysates and culture supernatant samples: Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 Total, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

The MILLIPLEX^® MAP Rat Adipocyte Magnetic Bead Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysates and culture supernatant samples: Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 Total, and TNFα.

特異性

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

應用

• Analytes: Adiponectin, IL-1β, IL-6, Leptin, MCP-1 (CCL2), PAI-1 (Total), TNFα
• Recommended Sample Type: Rat cell/tissue culture supernatant or lysates.
• Recommended Sample Dilution: 25 μL per well of cell/Tissue culture sample. Samples may require a dilution with an appropriate control medium.
• Assay Run Time: Overnight (16-18 hours) at 2-8°C
• Research Category: Metabolism
• Research Subcategory: Endocrine; Obesity; Metabolic Disease

特點和優勢

Design your multiplex kit by choosing available analytes within this panel.

包裝

Everything you need in a single kit.

成分

Rat Adipokine Standard, 1 vial, lyophilized

Rat Adipokine Quality Control 1, 1 vial, lyophilized

Rat Adipokine Quality Control 2, 1 vial, lyophilized

Assay Buffer, 1 bottle, 30 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Rat Adipocyte Detection Antibodies, 1 bottle, 5.5 mL

Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

儲存和穩定性

Recommended storage for kit components is 2 - 8°C.

其他說明

Please contact Technical Service for linearity of dilution.

Sensitivity: Please see kit protocol for individual assay sensitivities.

法律資訊

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 象形圖: GHS06,GHS09
• 訊號詞: Danger
• 危險聲明: H302 + H332,H311,H317,H319,H411
• 防範說明: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• 危險分類: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
• 儲存類別代碼: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects