OP143

Anti-MDM2 (Ab-3) Mouse mAb (4B11)

liquid, clone 4B11, Calbiochem^®

• 同義詞: Anti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Anti-Murine Double Minute Chromosome-2
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 4B11, monoclonal
• 形狀: liquid
• 不包含: preservative
• 物種活性: mouse, human
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 同型: IgG2a
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MDM2(4193) ; mouse ... Mdm2(17246)

描述

一般說明

Purified mouse monoclonal antibody. Recognizes the ~90 kDa (apparent MW) MDM2 protein.

Recognizes the ~90 kDa (apparent MW) MDM2 protein in A549 cells. May also recognize the ~60 kDa and ~90 kDa isoforms of MDM2.

This Anti-MDM2 (Ab-3) Mouse mAb (4B11) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MDM2 (Ab-3).

免疫原

Epitope: within amino acids 383-491

Human

full-length, recombinant human MDM2

應用

Immunoblotting (2 g/ml, chemiluminescence)
Immunofluorescence (1 g/ml)
Immunoprecipitation (1 g/reaction)
Paraffin Sections (2.5 g/ml, heat pre-treatment required)

包裝

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

外觀

In 50 mM sodium phosphate buffer, 50% glycerol.

重構

Following initial thaw, aliquot and freeze (-20°C).

分析報告

Positive Control
A549 cells

其他說明

Marchetti, A., et al. 1995. J. Pathol.175, 31.
Barak, Y., et al. 1993. EMBO. J.12, 461.
Ladanyi, M., et al. 1993. Cancer Res.53, 16.
Leach, F. S., et al. 1993. Cancer Res.53, 2231.
Oliner, J. D., et al. 1993. Nature362, 857.
Momand, J., et al. 1992. Cell69, 1237.
Oliner, J. D., et al. 1992. Nature358, 80.
Fakharzadeh, S. S., et al. 1991. EMBO. J.10, 1565.

May also recognize the ~60 kDa and ~90 kDa isoforms of MDM2. Antibody should be titrated for optimal results in individual systems.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3