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重要文件

NA70

Sigma-Aldrich

Anti-p14ARF (Ab-2) Mouse mAb (14PO2)

liquid, clone 14PO2, Calbiochem®

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About This Item

分類程式碼代碼:
12352203

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

14PO2, monoclonal

形狀

liquid

包含

≤0.1% sodium azide as preservative

物種活性

human

應無反應活性

rat, mouse

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

同型

IgG1

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

human ... CDKN2A(1029)

一般說明

Purified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splonocytes with mouse myeloma cells. Recognizes the ~14 kDa p14ARF protein.
Recognizes the ~14 kDa p14ARF protein in HeLa cells. Sold under license of U.S. Patent 6,482,929.
This Anti-p14ARF (Ab-2) Mouse mAb (14PO2) is validated for use in Immnunoblotting, Immunofluorescence, Immunoprecipitation for the detection of p14ARF (Ab-2).

免疫原

Human
recombinant, human p14ARF (p16β)

應用

Immnunoblotting (2-4 µg/ml)

Immunofluorescence (0.5 µg/ml)

Immunoprecipitation (2 µg/mg lysate)

包裝

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

外觀

In 10 mM PBS, 0.2% BSA, pH 7.4.

重構

Following initial thaw, aliquot and freeze (-20°C).

分析報告

Negative Control
Normal sheep serum
Positive Control
HeLa cells

其他說明

Antibody should be titrated for optimal results in individual systems.
Bates, S., et al. 1998. Nature395, 124.

法律資訊

Sold under license of U.S. Patent 6,482,929.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Stephen L Lessnick et al.
Cancer cell, 1(4), 393-401 (2002-06-28)
Ewing's sarcoma is associated with a fusion between the EWS and FLI1 genes, forming an EWS/FLI fusion protein. We developed a system for the identification of cooperative mutations in this tumor through expression of EWS/FLI in primary human fibroblasts. Gene
Chongwei Xie et al.
FEBS letters, 586(4), 435-441 (2012-01-31)
ARF is the second most commonly inactivated tumor suppressor behind p53. It has been implicated in the control of cell proliferation, cell senescence, and tumor suppression. However, the detailed mechanism underlying the transcriptional control of ARF remains largely unknown. Here

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