生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
14PO2, monoclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
human
應無反應活性
rat, mouse
製造商/商標名
Calbiochem®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
同型
IgG1
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... CDKN2A(1029)
一般說明
Purified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splonocytes with mouse myeloma cells. Recognizes the ~14 kDa p14ARF protein.
Recognizes the ~14 kDa p14ARF protein in HeLa cells. Sold under license of U.S. Patent 6,482,929.
This Anti-p14ARF (Ab-2) Mouse mAb (14PO2) is validated for use in Immnunoblotting, Immunofluorescence, Immunoprecipitation for the detection of p14ARF (Ab-2).
免疫原
Human
recombinant, human p14ARF (p16β)
應用
Immnunoblotting (2-4 µg/ml)
Immunofluorescence (0.5 µg/ml)
Immunoprecipitation (2 µg/mg lysate)
Immunofluorescence (0.5 µg/ml)
Immunoprecipitation (2 µg/mg lysate)
包裝
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外觀
In 10 mM PBS, 0.2% BSA, pH 7.4.
重構
Following initial thaw, aliquot and freeze (-20°C).
分析報告
Negative Control
Normal sheep serum
Normal sheep serum
Positive Control
HeLa cells
HeLa cells
其他說明
Antibody should be titrated for optimal results in individual systems.
Bates, S., et al. 1998. Nature395, 124.
法律資訊
Sold under license of U.S. Patent 6,482,929.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
Stephen L Lessnick et al.
Cancer cell, 1(4), 393-401 (2002-06-28)
Ewing's sarcoma is associated with a fusion between the EWS and FLI1 genes, forming an EWS/FLI fusion protein. We developed a system for the identification of cooperative mutations in this tumor through expression of EWS/FLI in primary human fibroblasts. Gene
Chongwei Xie et al.
FEBS letters, 586(4), 435-441 (2012-01-31)
ARF is the second most commonly inactivated tumor suppressor behind p53. It has been implicated in the control of cell proliferation, cell senescence, and tumor suppression. However, the detailed mechanism underlying the transcriptional control of ARF remains largely unknown. Here
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